Molecular Cell Biology. Purifying Proteins Cooper. References. Nelson and Cox, Lehninger’s Principles of Biochemistry, 3rd edition, pp. 130-137. Wikipedia: en.wikipedia.org/wiki/Protein_purification Lecture Presentation by Dr. Chrisopher Fraser: mcb.berkeley.edu/courses/mcb102/.
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• Preserve the structure during purification
• Consider that the structure may be lost
• Activity assay a good test
Using SDS-PAGE to Assess Purity
Separate the Polypeptides
Based on Mass