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Quiz 7

Results from Pinocytosis. Time

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Quiz 7

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    1. Quiz 7 The Enzyme used in the pinocytosis uptake was_______________________. This enzyme belongs to _______________ superfamily. The substrate to the enzyme is _________________________. The color of the substrate is _______________ and the product gives a ___________ color. spectrophotometer higher concentration of solution gives more absorbance /OD [T/F] Antigen is _______________________________________________ Antibody is __________________________________________ Immunofluorescence helps to detect presence of protein [T/F] Name two types of Immunofluorescence Antigen/ Antibody are nothing but proteins [T/F]

    2. Results from Pinocytosis Time              0'              5'                 15'                  30'   Absorbance    0            0.45              0.78               0.985 Color              white        yellow          orange     Light brown

    3. Cell Biology Lab 8 Immuno-fluorescence / IF PCB 4023 L TA: Sushmita Mustafi

    4. Objectives: Antigen/antibody Polyclonal/monoclonal antibody Primary/secondary antibody Immuno fluorescence Experiment protocol

    5. Antigen: Antigens are foreign to the host animal in which they produce a response. [immune response] A protein or polysaccharide part of bacteria/ viruses or other micro-organisms. Responsible for generating antibodies in the host.

    6. Antibody Antibody [Ag] : Immunoglobulins [ Ig] Gamma globulin type of proteins raised in blood or body fluids of vertebrates against antigen.

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