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VDX Hampton Research Hanging Drop 750uL Reservoir Volume. Q-Plate Hampton Research Sitting Drop 1mL Reservoir Volume. Q-Plate II Hampton Research Sitting Drop 750uL Reservoir Volume. Cryschem Hampton Research Sitting Drop 500uL Reservoir Volume. CombiClover Emerald BioStructures

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Presentation Transcript

  • VDX

  • Hampton Research

  • Hanging Drop

  • 750uL Reservoir Volume

  • Q-Plate

  • Hampton Research

  • Sitting Drop

  • 1mL Reservoir Volume

  • Q-Plate II

  • Hampton Research

  • Sitting Drop

  • 750uL Reservoir Volume

  • Cryschem

  • Hampton Research

  • Sitting Drop

  • 500uL Reservoir Volume

  • CombiClover

  • Emerald BioStructures

  • Sitting Drop

  • 750uL Reservoir Volume

Conclusions

  • Plate Type Experiment

    • Combiclover plate gave the most crystal hits over all proteins

    • 14 conditions gave hits with the Combiclover plate and no others

    • 3 conditions gave hits with Q-plate or Q-plate II and no others

    • 2 conditions gave hits with all trays except VDX and Combiclover

    • For only 5 conditions (across all proteins) did all 5 trays give hits

  • Crystallization Scan Experiment

    • Hampton Research Crystal Screen I had the best overall crystallization rate of success

    • Overall, there was a ~5-15% chance of crystallizing a protein from any one of the scans

    • Only 11 conditions (from a total of 336) yielded crystals of 2 or more of the 4 proteins tested

Exploring two variables in vapor diffusion crystallization: plate types and crystallization scans

Jennifer Pawlitz, Stephanie Shieh, Jennifer Sharamitaro, Eric Sturman, Mairi Lough, Anna Stevens

Discovery ResearchPharmacia Corporation Chesterfield Village, St. Louis, Missouri

Description of Plate Types

Description of Crystallization Scans

RAMC 2001 – Pharmacia Corporation Company Confidential!


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