1 / 8

海玉琰 2012.11.13

PEG200 浓度的测定. 海玉琰 2012.11.13. 实验步骤. 1 、配制 1.0 、 3.0 、 5.0 、 7.0 、 9.0 mg/L PEG 溶液. 分别取 0.1 、 0.3 、 0.5 、 0.7 、 0.9 mL 于 100mL 容量瓶，稀释至刻度，摇匀. 稀释至刻度 , 摇匀 , 得 1.0g/L 的母液. PEG200 1.0g. 容量瓶 1000mL. 2 、制作标准曲线. 不同浓度 PEG 溶液 5mL. 以 PEG 浓度为横坐标，光密度为纵坐标作图，制成标准曲线. 放置一定时间 以蒸馏水为空白 用分光光度计

gisela-lancaster
Download Presentation

海玉琰 2012.11.13

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PEG200浓度的测定 海玉琰 2012.11.13

  2. 实验步骤 1、配制1.0、3.0、5.0、7.0、9.0 mg/L PEG溶液 分别取0.1、0.3、0.5、0.7、0.9 mL于100mL容量瓶，稀释至刻度，摇匀 稀释至刻度,摇匀,得1.0g/L 的母液 PEG200 1.0g 容量瓶 1000mL 2、制作标准曲线 不同浓度 PEG溶液 5mL 以PEG浓度为横坐标，光密度为纵坐标作图，制成标准曲线 放置一定时间 以蒸馏水为空白 用分光光度计 测定光密度 10mL容量瓶， 稀释至刻度，摇匀 得0.5、1.5、2.5、3.5、4.5 mg/L PEG溶液 5% BaCl2溶液 1mL 0.02mol/L KI-I2试剂 1mL

  3. 溶液稀释50倍，测定其光密度 文献中在500nm左右测定PEG光密度

  4. A-t变化曲线 配制后5min左右测定

  5. A-W变化曲线

  6. 结果与讨论 • PEG-I2沉淀是一种极细的颗粒状沉淀物，呈棕色，沉降速度极慢。 • 碘测定PEG的方法可用于测定分子量为4000~20000的PEG[1-2]。 • PEGs of 3 different molecular weights (200, 400 and 600 Da) were employed in filtration tests. PEG concentrations were determined by gel permeation chromatography (GPC) performed on a Varian Inc. [3]. • The MW of samples from the feed and the collected permeate were analyzed via gel permeation chromatography. The GPC column HEMA Bio, linear, 10 μm, 600/8 mm, 100–3000000 g/mol, MZ, Germany, for the analysis of PEG solutions[4]. [1] Determination of polyethylene glycols by precipitation with iodine. Talanta,1985, 32(6):491-494. [2] 碘沉淀法测定聚乙二醇. 水处理技术, 1989, 15(5): 299-301. [3] Effects of hypochlorous acid exposure on the rejection of salt, polyethylene glycols, boron and arsenic(V) by nanofiltration and reverse osmosis membranes. Water Research, 2012, 46(16): 5217-5223. [4] Factors affecting the sieving behavior of anti-fouling thin-layer cross-linked hydrogel polyethersulfone composite ultrafiltration membranes. Journal of Membrane Science, 2012, 390-391: 99-112.

  7. 下一步计划 • 碘测定方法不适用 • 凝胶渗透色谱法 • 测出的数值(分子量、分子量分布等)如何换算成浓度？？ • 总有机碳(TOC)分析仪 可行

  8. Thank you !

More Related