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100 nM Paclitaxel. 0 4 8 12 24 48 (hr). Cleaved Caspase-9. Caspase-8. MCF-7. Figure S1a. **. *. MCF-7. Figure S1b. Paclitaxel 100 nM. - + - + - +. Bad siRNA. - - + + - -. Scrambled siRNA.

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100 nM Paclitaxel

0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

MCF-7

Figure S1a


**

*

MCF-7

Figure S1b


Paclitaxel 100 nM

- + - + - +

Bad siRNA

- - + + - -

Scrambled siRNA

- - - - + +

Bad

Actin

BimEL

BimL

IP: Bcl-2

IB: Bim

BimS

IP: Bcl-2

IB: Bcl-2

Bcl-2

Input

IB: Bcl-2

Bcl-2

Actin

MCF-7

Figure S1c


100 nM Paclitaxel

0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

T47D

Cleaved Caspase-3

Figure S1d


100 nM Paclitaxel

0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

MDA-MB-468

Cleaved Caspase-3

Figure S1e


100 nM Paclitaxel

0 4 8 12 24 48 (hr)

Cleaved Caspase-9

Caspase-8

BT20

Cleaved Caspase-3

Figure S1f


Paclitaxel 100 nM

0 4 8 12 [h]

Bim EL

IP: Bcl-xL

IB: Bim

Bim L

Bim S

IP: Bcl-xL

IB: Bcl-xL

Bcl-xL

MCF-7

Paclitaxel 100 nM

0 4 8 12 [h]

Bim EL

IP: Mcl-1

IB: Bim

Bim L

Bim S

IP: Mcl-1

IB: Mcl-1

Mcl-1

Figure S2a


Pre-IP

Post-IP

Untreated

Taxol 12 h

Untreated

HM

LM

S-100

HM

LM

S-100

Pre-IP

Post-IP

Paclitaxel 100 nM

BimEL

BimL

- +

BimEL

BimS

BimEL

BimL

BimL

BimS

BimS

CoxIV

Ratio

1 1.39

Ratio

1 0.43

BiP/Grp78

Actin

Actin

MCF-7

MCF-7

MCF-7

Figure S2b


Paclitaxel 100 nM

Paclitaxel 100 nM

MCF-7

MCF-7 Bcl-2+

MCF-7

MCF-7 Bcl-2+

- + - +

- + - +

IP: FLAG

IB: Bak

IP: FLAG

IB: Bcl-2

Bak

FLAG-Bcl-2

IP: FLAG

IB: Bax

FLAG-Bcl-2

Input

IB: Bcl-2

Bax

Bcl-2

IP: FLAG

IB: Puma

Puma

BimEL

IP: FLAG

IB: Bim

BimL

IP: FLAG

IB: Bad

BimS

Bad

*

BimEL

BimL

Input

IB: Bim

BimS

IP: FLAG

IB: Bmf

Bmf

Input

IB: Bmf

Bmf

Actin

Figure S2c


Paclitaxel 100 nM

- +

IP: Bcl-2

IB: Bax

Bax

IP: Bcl-2

IB: Bak

Bak

Paclitaxel 100 nM

- +

IP: Bcl-xL

IB: Bax

MCF-7

Bax

IP: Bcl-xL

IB: Bak

Bak

Paclitaxel 100 nM

- +

IP: Mcl-1

IB: Bax

Bax

IP: Mcl-1

IB: Bak

Bak

Figure S2d


Cyto

Mito

0 4 8 12 24 48

0 4 8 12 24 48 (hr)

Cytochrome c

T47D

CoxIV

Figure S3a


Cyto

Mito

0 4 8 12 24 48

0 4 8 12 24 48 (hr)

Cytochrome c

MDA-MB-468

CoxIV

Figure S3b


Cyto

Mito

0 4 8 12 24 48

0 4 8 12 24 48 (hr)

Cytochrome c

BT20

CoxIV

Figure S3c


100 nM Paclitaxel

0 4 8 12 24 48 (hr)

IP: Bax 6a7

IB: Bax N20

Bax

Bak

IP: Bak Ab-2

IB: Bak G23

Input

IB: Bax N20

Bax

Input

IB: Bak G23

Bak

Actin

T47D

Figure S4a


100 nM Paclitaxel

0 4 8 12 24 48 (hr)

IP: Bax 6a7

IB: Bax N20

Bax

IP: Bak Ab-2

IB: Bak G23

Bak

Input

IB: Bax N20

Bax

Input

IB: Bak G23

Bak

Actin

MDA-MB-468

Figure S4b


100 nM Paclitaxel

0 4 8 12 24 48 (hr)

Bax

IP: Bax 6a7

IB: Bax N20

IP: Bak Ab-2

IB: Bak G23

Bak

Input

IB: Bax N20

Bax

Input

IB: Bak G23

Bak

Actin

BT20

Figure S4c


Paclitaxel 100 nM

- + - + - +

Puma siRNA

- - + + - -

Scrambled siRNA

- - - - + +

Puma

Actin

MDA-MB-468

Figure S5a


Paclitaxel 100 nM

- + - + - +

Puma siRNA

- - + + - -

Scrambled siRNA

- - - - + +

Puma

Actin

BT20

Figure S5b


Paclitaxel 100 nM

- + - + - +

Puma siRNA

- - + + - -

Scrambled siRNA

- - - - + +

Puma

Actin

MCF-7

Figure S5c


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