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Cell Adhesion

Cell Adhesion. Matt Tanke Will Howerton Stewart Bewley Jeff Mills. Cell Adhesion Measurement Device. Cell sits between two plates One plate fixed, one plate attached to linear spring Actuator pulls spring into tension. Adhesion of Cell. Cell will have a smooth outer membrane or flagellum

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Cell Adhesion

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  1. Cell Adhesion Matt Tanke Will Howerton Stewart Bewley Jeff Mills

  2. Cell Adhesion Measurement Device • Cell sits between two plates • One plate fixed, one plate attached to linear spring • Actuator pulls spring into tension

  3. Adhesion of Cell • Cell will have a smooth outer membrane or flagellum • Both exhibit non-linear cell/plate separation Flagellum Smooth Membrane http://fig.cox.miami.edu/Faculty/Dana/105F00_4.html http://library.thinkquest.org/16985/cellmain.htm

  4. Adhesion of Cell

  5. Current Design: Optical Tweezers • Nm displacement • pN force • Glass bead dependent

  6. Scratch Drive • μN forces • 500 μm displacement • Difficult to model

  7. Nano-tractor • 40 nm step size • Moves at 3 mm/s • Stalls when 2.5 mN max tangential force is reached

  8. How it works

  9. Accuracy

  10. SUMMiT V Process Flow • Conformal Deposition • Planar Depostion • Dry Etch • Wet Etch • Release Etch

  11. Diagram and Variables ks kc D1 D2 P2 P1 • 6 variables: D2, P2, ks, D1, P1, kc • 2 equations can be derived: • P1 = D1*(kc + ks) – D2*ks • P2 = ks*(D2 – D1) • kc is the spring constant of the cell • ks is the spring constant of the spring • D2 is controlled by nano-tractor • P2 is the force needed for nano-tractor • P1 is the force needed to pull the cell • D1 is the displacement of the cell

  12. P2 is not needed Assumptions need to be made for other variables in order to find values for k’s Finding kc Assumptions Cell is a cylinder Diameter equals length Diameter roughly 10-50 um Calculation kc = E*pi*d/4 kc = .0076-.0393 N/m Assumptions ks kc D1 D2 P2 P1

  13. Assumptions P1 = 1-100 nN D2 = 11 um – ls + D1 Due to limit of optical measuring tool Assume ls = 8 um Made spreadsheet ks = 0.0246 N/m Verification Find increment for D2 that works for range Must be greater than 40 nm Selected 250 nm Check if increment will be detectable Finding ks ks kc D1 D2 P2 P1

  14. Overview of Range

  15. Spring Dimensions • 2 double cantilever beams • ks = E*t*(w/l)3 • w = 2.25 um • l = 785 um • T = 7 um

  16. Results

  17. Thank You Questions?

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