Analisa asam lemak dgn glc
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ANALISA ASAM LEMAK DGN GLC. PROF. SBW. PRINSIP ANALISA. MINYAK KACANG/SAMPEL DILARUTKAN DALAM HEKSAN, DITAMBAHKAN LARUTAN KOH METANOLAT, AGAR TERJADI METIL ESTER, CAMPURAN DISENTRIFUS, SUPERNATAN YANG MENGANDUNG METIL ESTER SIAP DIINJEKSIKAN KE GLC. PRINSIP KERJA.

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ANALISA ASAM LEMAK DGN GLC

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ANALISA ASAM LEMAK DGN GLC

PROF. SBW


PRINSIP ANALISA

  • MINYAK KACANG/SAMPEL DILARUTKAN DALAM HEKSAN, DITAMBAHKAN LARUTAN KOH METANOLAT, AGAR TERJADI METIL ESTER, CAMPURAN DISENTRIFUS, SUPERNATAN YANG MENGANDUNG METIL ESTER SIAP DIINJEKSIKAN KE GLC.


PRINSIP KERJA

  • REAGEN: LAR 2 N KOH METANOLAT DAN HEKSAN

  • TAHAP PERSIAPAN SAMPEL:

  • TIMBANG MINYAK TEPAT ( 10 mg) larutkandalam 2 ml Heksan, tambahkan 0,2 ml @ M KOH metanolat, Vortex campurantsb, sentrifusdgnkecepatanttt (3000 rpm) selama 5 menit. Supernatanmengandungmetil ester siapdiinjeksikankedalam GLC.


KONDISI MESIN GLC

  • GLC Hewlett-Packard model 5170 A detektor FID.

  • Kolom stainless steel 6 ft internal diameter 1/8 inch, diisi 10% DEGS pada 80-100 mesh Chromosorb W/AW

  • FASE MOBIL: GAS N2 20 ml/menit, H2 20 ml/menit

  • SUHU INJEKTOR 250 OC, SUHU DETEKTOR 300 OC DAN SUHU KOLOM 195 OC

  • Sampeldiinjeksikan 1,2 uL

  • Kfromatogram yang dihasilkansbb:


Kromatogrammetil ester asamlemakminyakkacang


Perhitungankomponenfraksi per sampeldgnlarutanstandarteksternal

  • PRINSIP PERHITUNGAN:

  • KADAR STD (STANDART) LUAS AREA STD

  • KADAR KOMPONEN SAMPEL LUAS AREA KOMPONEN SAMPEL

  • KADAR KOMPONEN Unknown sampel =

  • Kadar STD X Luas area Komponen Unknown

  • Luas area STD


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