Streptococci
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Streptococci. Colonial morphology Microscopic appearance Lancefield grouping - How to distinguish between Streptococci. Streptococci. Streptococcus pneumoniae. Streptococcus pneumoniae.

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Streptococci

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Streptococci

Colonial morphology

Microscopic appearance

Lancefield grouping

- How to distinguish between

Streptococci


Streptococci


Streptococcuspneumoniae


Streptococcuspneumoniae


- On Blood Agar: Translucent or mucoid colonies, 1-2 mm in diameter, raised colonies in young cultures but later become flattened with raised edges.


Bile Solubility Test


Optochin Test


Streptococcuspyogenes

- On Blood Agar: small 0.5 – 1 mm in diameter colourless colonies, dry, shiny or mucoid beta haemolytic colonies.


Streptococcusagalactiae


- On Blood Agar: grey mucoid colonies, 2mm in diameter surrounded by small zone of Beta-haemolysis.


CAMP Test

- CAMP factor is an extracelluar diffusible protein produced by S.agalactiae

- This protein interacts with the staphylococcal beta-lysin on sheep blood agar producing enhanced haemolysis.

- arrow-head shaped area of haemolysis where the staphylococcal organism meets the test organism.


Enterococcusfaecalis


On Blood Agar: non-haemolytic colonies.


  • Aesculinis incorporated into agar with ferric citrate and bile salts

  • bile aesculin agar.

  • Hydrolysis of the aesculin forms aesculetinand glucose.

  • The aesculetin forms dark brown or black complexes with ferric citrate.


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