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Toxicity of Automotive Runoff on Daphnia. Morgan Dutrow 10 th Grade Bellwood-Antis. Why I Chose this Experiment. I chose this experiment because…. Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments.

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toxicity of automotive runoff on daphnia

Toxicity of Automotive Runoff on Daphnia.

Morgan Dutrow

10th Grade

Bellwood-Antis

why i chose this experiment
Why I Chose this Experiment

I chose this experiment because…

  • Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments.
  • Earlier in the year I had learned a little about Daphnia.
slide3

Background Information

  • Brake fluid
  • Contains: Diethylene glycol (C4O3H)

Windshield Washer Fluid

  • Contains: Methanol (CHO3H)
  • Antifreeze
  • Contains: Ethylene glycol (C2H6O2)

Lethal Dose 50 in 96 hours

hypothesis
Hypothesis

Windshield washer fluid be the least lethal.

why i chose daphnia
Why I Chose Daphnia…
  • In our experiment we used the variety of daphnia known as Daphnia Magnum.
  • Daphnia being much smaller, would be more susceptible. Because they are a food source, this could then work its way up the ecosystem.
  • Daphnia are an indicator species.
general procedure
General Procedure
  • 40ml fluid with 4 daphnia
  • 3 trials of each dilution
making a dilution ex 1 10 dilution
Making a dilution: Ex. 1/10 dilution
  • Water: 34ml
  • Automotive pollutant: 4ml
  • Food:2ml
recipes for my dilutions
Recipes For My Dilutions

Dilution Water Food Chemical

1/2 18 2 20

1/5 30 2 8

1/10 34 2 4

1/20 36 2 2

1/40 37 2 1

1/80 37 ½ 2 ½

experimental design
Experimental Design
  • Variable
    • the pollutant
  • Experimental Group
    • Antifreeze, Brake Fluid, and Windshield Washer Fluid
  • Control Group
    • Spring Water
experimental design contd
Experimental Design Contd.
  • Control Variables:

Amount of…

    • Light
    • Food
    • Chemical
    • Water

Others…

    • Temperature
    • Size of daphnia
    • Clean materials
ld 50
LD 50
  • Daphnia died too close together
averages daphnia lifetimes of 3 trials
Averages Daphnia Lifetimes of 3 Trials

Note: All numbers are in minutes.

conclusion
Conclusion

According to my data, I reject my hypothesis that Windshield Washer Fluid would be the least lethal to daphnia.

improvements
Improvements
  • I would…
    • use more specific dilutions.
    • use different animals.
    • use more chemicals.
expanding my project
Expanding my Project
  • I could…
    • test motor oil by using a larger aquatic animal.
    • test to see what affect making the water more acidic or basic would have.
bibliography
Bibliography
  • http://ijt.sagepub.com/cgi/content/abstract/23/1/55 1/24
  • http://www.aehms.org/jaeh_3_2_sherry.html 1/24
  • http://www.jtbaker.com/msds/englishhtml/e5125.htm 2/8
  • https://fscimage.fishersci.com/msds/89493.htm 2/8
  • http://www.atsdr.cdc.gov/tfacts96.html 2/10
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