The effects of caffeine on the heart rate of daphnia magna
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The Effects of Caffeine on the Heart Rate of Daphnia magna. Laura Haggerty. Question. How does the introduction of caffeine affect the heart rate of Daphnia magna?. Background Information. Daphnia magna are minute water crustaceans commonly known as water fleas.

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The Effects of Caffeine on the Heart Rate of Daphnia magna

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The effects of caffeine on the heart rate of daphnia magna

The Effects of Caffeine on the Heart Rate of Daphnia magna

Laura Haggerty


Question

Question

How does the introduction of caffeine affect the heart rate of Daphnia magna?


Background information

Background Information

  • Daphnia magna are minute water crustaceans commonly known as water fleas.

    • Their skin is transparent making all internal organs visible under microscope

  • Typical life span is approximately 40-56 days

    • Environmental factors and temperature can alter the life span of Daphnia magna.

  • Caffeine (C8H10N4O2 ) is a stimulant drug

  • Heart rate is defined as the number of beats per minute


Hypothesis

Hypothesis

It was hypothesized that as Daphnia magna were introduced to increasing concentrations of caffeine, their heart rate would also increase


Variables

Variables

Independent variable- concentration of caffeine

Dependent variable- heart rate of Daphnia magna

Constants- spring water

Control- spring water with no added caffeine


Materials

Materials

Daphnia magna

Transfer pipettes

100 mL graduated cylinders (2)

5 petri dishes

Compound microscope

Depression slides

Small container

250 mL beakers (5)

Caffeine pill (200 mg)

Detain


Procedure

Procedure

Serial dilutions were made in five 250 mL beakers

This resulted in a control and four varying concentrations of 1 mg/ml, 0.1 mg/ml, 0.01 mg/ml, and .001 mg/ml

The Daphnia magna were placed in each solution in small petri dishes before being transferred to depression slides.

The depression slides were placed on a compound microscope and observed.

The heart rate of the Daphnia magna was then counted for ten seconds and multiplied by six to calculate the results for heart rate per minute.

Five trials were performed for each of the four concentrations and the control.


The effects of caffeine on the heart rate of daphnia magna

Data


The effects of caffeine on the heart rate of daphnia magna

Heartbeats per minute


Analysis

Analysis

  • The hypothesis was supported. When introduced to higher concentrations of caffeine, the heart rate of Daphnia magna increased.

  • The two most concentrated solutions proved to be significant exhibiting the fact that caffeine does affect the heart rate of the Daphnia, but the solution must be concentrated to a certain level in order for a distinct variation in heart rate to occur.

  • For further research, one might extend the experiment to test other small organisms

  • Another extension could be to perform additional trials with more concentrations to pinpoint the exact concentration at which toxicity occurs.


Conclusion

Conclusion

  • The introduction of caffeine had a substantive effect on the heart rate of Daphnia magna tested thus supporting the hypothesis that an increase in caffeine concentration would instigate an increase in heart rate.

    • The more concentrated solutions of .1 mg/ml and higher were shown to have had a significant effect on the heart rate.


Works cited

Works Cited


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