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Conservation Status and Phylogeography of Cowhead Lake Tui Chub in the Northwestern Great Basin

Conservation Status and Phylogeography of Cowhead Lake Tui Chub in the Northwestern Great Basin. Yongjiu Chen & Bernie May Genomic Variation Laboratory Department of Animal Science University of California, Davis, CA 95616 Stewart Reid, 2045 East Main St. Ashland, OR 97520. Background.

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Conservation Status and Phylogeography of Cowhead Lake Tui Chub in the Northwestern Great Basin

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  1. Conservation Status and Phylogeography of Cowhead Lake Tui Chub in the Northwestern Great Basin Yongjiu Chen & Bernie May Genomic Variation Laboratory Department of Animal Science University of California, Davis, CA 95616 Stewart Reid, 2045 East Main St. Ashland, OR 97520

  2. Background • Small fish in the minnow family (Cyprinidae). • Subspecies, Siphateles bicolor vaccaceps. • Cowhead Lake basin, Modoc Co, California. • Its genetic relationship is uncertain. • Population decline due to increased human activity and natural stochastic events. • Proposed for listing under the US ESA in 1998.

  3. Cowhead Lake Slough

  4. Questions • Is the Cowhead Lake tui chub distinct from other geographically proximate populations? • Which tui chub population is the most closely related? • What is the degree of genetic variation remaining in the Cowhead Lake tui chub population?

  5. Materials & Methods • Two independent tui chub samplings from the Cowhead Lake. • Assess genetic variability at the population level. • Use 10 microsatellite DNA loci. High variation within populations Bi-parentally inherited

  6. Summer Lake Catlow Valley Oregon NORTH AMERICA Warner Valley Goose Lake Cowhead Lake Pit River Nevada California Eagle Lake Sample Localities

  7. Cowhead Lake Tui Chub Goose Lake Tui Chub Gel Image of Cowhead Lake & Goose Lake tui chubs

  8. Comparison of genetic variability

  9. Pairwise FST and genetic differentiation

  10. Catlow Valley Summer Lake Cowhead Lake Warner Valley Goose Lake/Pit River Eagle Lake Factorial correspondence analysis

  11. Catlow Valley Summer Lake Eagle Lake Cowhead Lake Pit River Warner Valley Goose Lake 0.1 FST-Based Neighbor-Joining Tree

  12. Warner Valley(37) Eagle Lake(33) Summer Lake(69) Goose Lake/Pit River(53) Cowhead Lake(79) Catlow Valley(15) Bayesian analysis of individual assignment

  13. Conclusions • The Cowhead Lake tui chub is distinct from other geographically proximate populations. • No substantial differentiation within the remnant Cowhead Lake population. • Comprises relatively low degrees of genetic variability. • Should be listed as “endangered” under the US ESA. • The Warner Valley tui chub may be the most closely related to the Cowhead. • Goose Lake and Pit River tui chubs are the most closely related.

  14. Future Studies • Population structure of Cowhead Lake tui chub. • Gene flow between Cowhead Lake and Warner Valley tui chub populations. • Genetic and taxonomic status of Summer Lake and Eagle Lake tui chubs.

  15. Acknowledgement Dr. Douglas Markle, Oregon State University Dr. Peter Moyle, UC Davis Genomic Variation Laboratory, UC Davis California Department of Fish & Game U. S. Fish & Wildlife Service

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