MOLECULAR RADAR

1. MOLECULAR RADAR Forensic Analysis at the Molecular Level Presentation for FLC Mid-Atlantic Region 21 September 2006 R. Paul Schaudies, Ph.D. Doreen A. Robinson, Ph.D. 703-298-3720 SAIC ProprietarrySAIC Proprietary – Competition Sensitive

2. Molecular RadarTM Biological Forensic Identification Technology • Highly multiplexed nucleic acid hybridization based approach • Target only unique and informative genetic regions • Microarray format allows for identification of tens of thousands of individual sequences in parallel • “Complete” genetic characterization within 4-24 hours • Customizable levels of resolution SAIC ProprietarrySAIC Proprietary – Competition Sensitive

3. Capabilities Offered • Functional equivalent of a 10,000-fold multiplexed PCR reaction • Simultaneously identify multiple organisms • Strain level resolution • Identify virulence factors • Identify antibiotic resistance genes • Identify signs of genetic engineering • Characterize unknown organisms • No sample dilution for multiple analyses • Technology is adaptable to multiple platforms and applications SAIC ProprietarrySAIC Proprietary – Competition Sensitive

4. System Concept Assay Development Routine Sample Analysis • Computer identification of informative DNA/RNA sequences • Identification of candidate oligonucleotides • On-chip synthesis of oligos • Whole-genome amplification with label incorporation • Hybridization on chip • Spot profile identifies sequences present in original sample SAIC ProprietarrySAIC Proprietary – Competition Sensitive

5. B anthracis Sterne B anthracis Ames Bacillus anthracis Ames vs Sterne on SAIC VER 1 Array SAIC ProprietarrySAIC Proprietary – Competition Sensitive

6. SAIC VER1 Array Hybridizations SAIC ProprietarrySAIC Proprietary – Competition Sensitive

7. Three-Dimensional Data Presentation • Three hybridizations using identical arrays • Each data point represents the performance of a specific oligonucleotide in three separate hybridizations • Unique sequences fall on the axes • Conserved sequences fall on the planes or at the top center if present in all three organisms SAIC ProprietarrySAIC Proprietary – Competition Sensitive

8. Virulence Factors for Bacillus # of Spots 2 Virulence B. anthracis Ames # of Spots 16 Virulence 313 pX01 47 Genomic # of Spots 10 Virulence 2 mRNA 2 Genomic B. cereus G9421 B. thuringiensis 97-27 # of Spots 17 Genomic SAIC ProprietarrySAIC Proprietary – Competition Sensitive

9. Virulence Factors for Yersinia Y. pestis CO92 # of Spots 6 Virulence 14 pCD1 92 Genomic # of Spots 16 Virulence 10 Genomic Y. enterocolitica 9610 Y. pseudotuberculosis 11960 SAIC ProprietarrySAIC Proprietary – Competition Sensitive

10. Summary • Molecular RadarTM provides high fidelity forensic identification and characterization of microorganisms • We have achieved resolution down to the level of strain for pathogens and near-neighbor organisms • We can design and validate arrays for any DNA or RNA containing organism at desired level of resolution SAIC ProprietarrySAIC Proprietary – Competition Sensitive

11. Contact Information • R. Paul Schaudies, Ph.D. • 703-298-3720 • schaudiesr@saic.com • Doreen A. Robinson, Ph.D. • 703-298-3720 • robinsond2@saic.com SAIC ProprietarrySAIC Proprietary – Competition Sensitive