MOLECULAR RADAR
This presentation is the property of its rightful owner.
Sponsored Links
1 / 11

MOLECULAR RADAR PowerPoint PPT Presentation


  • 81 Views
  • Uploaded on
  • Presentation posted in: General

MOLECULAR RADAR. Forensic Analysis at the Molecular Level. Presentation for FLC Mid-Atlantic Region 21 September 2006. R. Paul Schaudies, Ph.D. Doreen A. Robinson, Ph.D. 703-298-3720. Molecular Radar TM Biological Forensic Identification Technology.

Download Presentation

MOLECULAR RADAR

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Molecular radar

MOLECULAR RADAR

Forensic Analysis

at the Molecular Level

Presentation for

FLC Mid-Atlantic Region

21 September 2006

R. Paul Schaudies, Ph.D.

Doreen A. Robinson, Ph.D.

703-298-3720

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Molecular radar tm biological forensic identification technology

Molecular RadarTM Biological Forensic Identification Technology

  • Highly multiplexed nucleic acid hybridization based approach

  • Target only unique and informative genetic regions

  • Microarray format allows for identification of tens of thousands of individual sequences in parallel

  • “Complete” genetic characterization within 4-24 hours

  • Customizable levels of resolution

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Capabilities offered

Capabilities Offered

  • Functional equivalent of a 10,000-fold multiplexed PCR reaction

  • Simultaneously identify multiple organisms

  • Strain level resolution

  • Identify virulence factors

  • Identify antibiotic resistance genes

  • Identify signs of genetic engineering

  • Characterize unknown organisms

  • No sample dilution for multiple analyses

  • Technology is adaptable to multiple platforms and applications

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


System concept

System Concept

Assay Development

Routine Sample Analysis

  • Computer identification of informative DNA/RNA sequences

  • Identification of candidate oligonucleotides

  • On-chip synthesis of oligos

  • Whole-genome amplification with label incorporation

  • Hybridization on chip

  • Spot profile identifies sequences present in original sample

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Molecular radar

B anthracis Sterne

B anthracis Ames

Bacillus anthracis Ames vs Sterne on SAIC VER 1 Array

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Molecular radar

SAIC VER1 Array Hybridizations

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Three dimensional data presentation

Three-Dimensional Data Presentation

  • Three hybridizations using identical arrays

  • Each data point represents the performance of a specific oligonucleotide in three separate hybridizations

  • Unique sequences fall on the axes

  • Conserved sequences fall on the planes or at the top center if present in all three organisms

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Molecular radar

Virulence Factors for Bacillus

# of Spots

2 Virulence

B. anthracis Ames

# of Spots

16 Virulence

313 pX01

47 Genomic

# of Spots

10 Virulence

2 mRNA

2 Genomic

B. cereus G9421

B. thuringiensis 97-27

# of Spots

17 Genomic

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Molecular radar

Virulence Factors for Yersinia

Y. pestis CO92

# of Spots

6 Virulence

14 pCD1

92 Genomic

# of Spots

16 Virulence

10 Genomic

Y. enterocolitica 9610

Y. pseudotuberculosis 11960

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Summary

Summary

  • Molecular RadarTM provides high fidelity forensic identification and characterization of microorganisms

  • We have achieved resolution down to the level of strain for pathogens and near-neighbor organisms

  • We can design and validate arrays for any DNA or RNA containing organism at desired level of resolution

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


Contact information

Contact Information

  • R. Paul Schaudies, Ph.D.

    • 703-298-3720

    • [email protected]

  • Doreen A. Robinson, Ph.D.

    • 703-298-3720

    • [email protected]

SAIC ProprietarrySAIC Proprietary – Competition Sensitive


  • Login