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Cell cycle expression patterns are highly correlated in F2 islets

A sub-group of transcripts that are positively correlated. Cell cycle expression patterns are highly correlated in F2 islets. A sub-group of transcripts that are positively correlated. F2 mice are stratified by cell cycle expression patterns. lower insulin higher glucose lower # islets

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Cell cycle expression patterns are highly correlated in F2 islets

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  1. A sub-group of transcripts that are positively correlated Cell cycle expression patterns are highly correlated in F2 islets

  2. A sub-group of transcripts that are positively correlated F2 mice are stratified by cell cycle expression patterns lower insulin higher glucose lower # islets higher TG lower body weight lower c-peptide “non-diabetic” “diabetic”

  3. Distribution of a few cell cycle genes in F2 islets Is this difference in the expression of cell cycle genes sufficient to play a role in causing diabetes in the F2 mice?

  4. Islet genes with “Regulation of progression through cell cycle” GO term Genome-wide linkage of cell cycle regulatory transcripts

  5. Adipose genes with “Regulation of progression through cell cycle” GO term Genome-wide linkage of cell cycle regulatory transcripts

  6. Islet genes with “Regulation of progression through cell cycle” GO term eQTL linkage on Chr 2 for cell cycle genes in islet

  7. Islet genes with “Regulation of progression through cell cycle” GO term eQTL linkage on Chr 2 for cell cycle genes in islet

  8. Islet genes with “Regulation of progression through cell cycle” GO term cis and trans eQTLs on Chr 2 for cell cycle regulation Gene Chr Mb Cdk5rap1 2 153.8 E2f1 2 154.0 Rbl1 (p107) 2 156.6 Igf2 7 137.1 Aip 19 39.0 LOD

  9. Ccnh Cdkn2b Ccnd1 Strong cell cycle linkages occur across the genome Pdgfb Foxa2 Cdk3 Rb1 Ccnk LOD

  10. body wt. # transcripts (divided by 20) with eQTL lod > 2

  11. cell cycle module Co-expression modules identified in female F2 islets F2 female islet network MDS plot

  12. Expression heat map for female F2 islets ~7500 transcripts in 14 modules cell cycle 228 female F2 mice

  13. Cell cycle genes common in time course and F2 female islet modules

  14. Genome-wide mapping of cell cycle gene set in F2 islets Nfx1 nuclear transcription factor, x-box binding 1 Chr 4, 41.1 Mb Prc1 protein regulator of cytokenesis 1 Chr 7, 80.1 Mb LOD Nrm nurim Chr 17, 35.4 Mb

  15. LOD rescaled for each transcript, p-value threshold = 0.05 Genome-wide mapping of cell cycle gene set in F2 islets

  16. LOD rescaled for each transcript, p-value threshold = 1.0 (will include all traits) Genome-wide mapping of cell cycle gene set in F2 islets

  17. LOD rescaled for each transcript, p-value threshold = 1.0 (will include all traits) Mapping of cell cycle gene set in F2 islets to putative “hot spots”

  18. LOD not rescaled for each transcript p-value threshold = 1.0 (will include all traits) Mapping other modules in female F2 islets F2 female islet network

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