Cell cycle
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Cell Cycle. Cell Cycle Control of DNA Replication Initiation of replication involves replicators and the origin recognition complex, or ORC , a heteromeric protein that binds to replicators. ORC is bound to the replicators throughout the cell cycle. Early in G1

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Cell Cycle

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Cell cycle

Cell Cycle


Cell cycle

Cell Cycle Control of DNA Replication

Initiation of replication involves replicators and the

origin recognition complex, or ORC, a heteromeric

protein that binds to replicators. ORC is bound to

the replicators throughout the cell cycle. Early in G1

(just after M), ORC serves as a “landing pad” for

proteins essential to replication control. Proteins binding

to ORC establish a pre-replication complex (pre-RC),

but the pre-RC can be formed only during a window

of opportunity during G1. One of the principal proteins

in assembly of the pre-RC in yeast is Cdc6p (the

replication activator protein encoded by the yeast

cdc6 gene). Once Cdc6p binds to ORC, replication

licensing factors (RLFs) that “license” or permit

DNA replication to occur then bind to the

chromosomes.

www.web.virginia.edu/Heidi/chapter30/chp30.htm


Cell cycle

Fluorescence Activated Cell Sorting (FACS)

http://www.bio.davidson.edu/COURSES/GENOMICS/method/FACS.html


Cell cycle

FLAG-Tag Plasmid (Example)

HA-Tag: (hemagglutinin)

YPYDVPDYA


Cell cycle

PCNA (proliferating cell nuclear antigen)

The protein encoded by this gene is found in the nucleus and is a cofactor of

DNA polymerase. The encoded protein acts as a homotrimer and helps

increase the processivity during DNA replication.

http://biochemie.web.med.uni-muenchen.de/biotutor_2004/replikat.htm


Cell cycle

DpnI cleaves DNA only when it is methylated on both strands.

And mammalian cells lack the ability to methyate Dpn I restriction sites.


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