1 / 16

Cell Cycle

Cell Cycle. Cell Cycle Control of DNA Replication Initiation of replication involves replicators and the origin recognition complex, or ORC , a heteromeric protein that binds to replicators. ORC is bound to the replicators throughout the cell cycle. Early in G1

gauri
Download Presentation

Cell Cycle

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Cell Cycle

  2. Cell Cycle Control of DNA Replication Initiation of replication involves replicators and the origin recognition complex, or ORC, a heteromeric protein that binds to replicators. ORC is bound to the replicators throughout the cell cycle. Early in G1 (just after M), ORC serves as a “landing pad” for proteins essential to replication control. Proteins binding to ORC establish a pre-replication complex (pre-RC), but the pre-RC can be formed only during a window of opportunity during G1. One of the principal proteins in assembly of the pre-RC in yeast is Cdc6p (the replication activator protein encoded by the yeast cdc6 gene). Once Cdc6p binds to ORC, replication licensing factors (RLFs) that “license” or permit DNA replication to occur then bind to the chromosomes. www.web.virginia.edu/Heidi/chapter30/chp30.htm

  3. Fluorescence Activated Cell Sorting (FACS) http://www.bio.davidson.edu/COURSES/GENOMICS/method/FACS.html

  4. FLAG-Tag Plasmid (Example) HA-Tag: (hemagglutinin) YPYDVPDYA

  5. PCNA (proliferating cell nuclear antigen) The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase. The encoded protein acts as a homotrimer and helps increase the processivity during DNA replication. http://biochemie.web.med.uni-muenchen.de/biotutor_2004/replikat.htm

  6. DpnI cleaves DNA only when it is methylated on both strands. And mammalian cells lack the ability to methyate Dpn I restriction sites.

More Related