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NIBSC Multiplex Proficiency Panel - A multicentre study

Clare Morris and David Padley Divisions of Retrovirology and Virology. NIBSC Multiplex Proficiency Panel - A multicentre study. Panel History. Panels produced for the UK National Blood Transfusion Authority. UK requirement to screen for HCV RNA in blood donations since 1999.

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NIBSC Multiplex Proficiency Panel - A multicentre study

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  1. Clare Morris and David Padley Divisions of Retrovirology and Virology NIBSC Multiplex Proficiency Panel - A multicentre study

  2. Panel History • Panels produced for the UK National Blood Transfusion Authority. • UK requirement to screen for HCV RNA in blood donations since 1999. • Initially, annual HCV proficiency panels produced to monitor assay and operator performance across UK sites. • In 2005 HIV-1 screening introduced in the one NBS site. • 1st HIV-1 RNA Proficiency panel produced in 2006. • Panels have consisted of dilutions of a single marker, up to 15 members/panel.

  3. New multiplex approach • Panel consisted of 15 coded samples. • Contained combinations of HIV, HCV, HBV. • One plasma negative was included. • 7 labs took part, 2 labs reported data using two systems. • Due to potential for confirmatory testing each lab was sent 4 panels. • Requested to test panel through routine algorithm. • 5 labs used commercial assays, 2 used in house assays

  4. Panel Details

  5. HIV-1 Results

  6. HCV Results

  7. HBV results

  8. Conclusions • HIV: One lab didn’t detect 100IU in triplex sample. All other labs detected HIV at all concentrations. • HCV: Lab 2 failed to detect 20 IU sample. Lab 6 failed to detect 10 IU sample & 100 IU in a triplex sample. • HBV: 3 data sets available. All HBV samples detected. • 50 proficiency panels remaining, if you would like to receive one please E mail me at: Clare.Morris@nibsc.hpa.org.uk

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