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Four Channels Data Acquisition System for Silicon Photomultipliers. Mateusz Baszczyk, Piotr Dorosz, Sebastian Głąb , Wojciech Kucewicz, Łukasz Mik, Maria Sapor. Department of Electronics, AGH – University of Science and Technology, Al. Mickiewicza 30, 30-059 Krakow, Poland
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Four Channels Data Acquisition System for Silicon Photomultipliers Mateusz Baszczyk, Piotr Dorosz, Sebastian Głąb,Wojciech Kucewicz, Łukasz Mik, Maria Sapor Department of Electronics, AGH –University of Science and Technology, Al. Mickiewicza 30, 30-059 Krakow, Poland e-mail kucewicz@agh.edu.pl sglab@agh.edu.pl
Agenda • Fluorescence; • Silicon Photomultiplier description; • Description of previous measurement system; • Four channel data acquisition system; • Results; • Summary; • Future work.
Why do we measure fluorescence light intensity? • Dye-labelled cells can be count by fluorescence light intensity measurement; • At low concentrations the fluorescence intensity is generally proportional to the concentrations of fluorophore.
Fluorescein and Bio-Tek filters spectra fluorescein resorufin
Silicon Photomultiplier • The idea appears in the late 90-ties: • „Photon Detection with High Gain Avalanche Photodiode Arrays” S. Vasile, P. Gothoskar, R. Farrell, D. Sdrulla • „Silicon avalanche photodiodes on the base of metal-resistor-semiconductor (MRS) structures” V. Saveliev, V. Golovin
Photodiode Operation Mode 1 photon generates 1 electron-hole pair
Avalanche Operation Mode 1 photon generates 10-200 electron-hole pairs
Geiger Operation Mode 1 photon generates 105 - 106 electron-hole pairs
Previous Measurement System For Fluorescence Agilent 33250 generator trigger signal gate pulses Picosecond 2600C generator Cuvette holder with specimen GPA amplifier QDC device PC with Labview application excitation filter emission filter
61.05 [pg/ml] 976.6 [pg/ml] Results of fluorescence light measurements y=-572500x²+223.6x+6458000 – fluorescein y=-373800 x²+170.3 x +14030000 – resorufin
Conclusions • We have designed, built and optimized readout system for detection of single photons; • We have tested readout circuit with light and confirmed that it is linear; • We can distinguish a dozen or so photons and eliminate thermal generation from measurement data; • System with silicon photomultiplier has a good sensitivity and can be used to measure fluorescence light intensity for fluorescein concentration from 1ug/ml to about 60pg/mland resorufin at the level of 1ng/ml up to 1ug/ml.
Future work • Stabilization system compensating temperature influence on SiPM gain by varying supply voltage; • Measurements of fluorescent dyes in microbioreactors. scanning probe micro-bioreactor