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Electronic Supplementary Material for the manuscript entitled:

Electronic Supplementary Material for the manuscript entitled: Effective selenium detoxification in the seed proteins of a hyperaccumulator plant: the analysis of selenium containing proteins of monkeypot nut ( Lecythis minor ) seeds

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Electronic Supplementary Material for the manuscript entitled:

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  1. Electronic Supplementary Material for the manuscript entitled: Effective selenium detoxification in the seed proteins of a hyperaccumulator plant: the analysis of selenium containing proteins of monkeypot nut (Lecythis minor) seeds by A. Németh and M. Dernovics, published inJournal of Biological Inorganic Chemistry Table S 1Chromatographic parameters applied. Table S2 Instrumental parameters of the HPLC-ESI-QTOF-MS set-up.

  2. Table S3. Database search and protein validation settings. Table S4.Protein database search identification results. The coverage was calculated by the sotware based on the sequence of the precursor protein. In the case of 2S albumin and 2S sulfur-rich proteins, the signal and propeptides are removed during the maturation of the proteines. The revised coverages were calculated based on the sequence of the mature proteins. Methionine coverage is the coverage of methionine containing peptides. Se-containing peptides are highlighted.

  3. (‘) Figure S 1. The MS-MS spectra and the identification details of the peptide GILGVLMPGCPETF and its selenium analogue. While the selenopeptide was identified by the search engines as a Sec containing peptide, the manual checking of the MS-MS spectra revealed the absence of Sec containing fragments (y5’, y6’, y7’). In the peptide sequence C* corresponds to carbamydomethyl-cysteine, B denotes to selenomethionine and Sec represents selenocysteine. x102 2 Intensity. cps 1 0 m/z 200 300 400 500 600 700 800 900 1000 780.4568 780.4568 828.4013 997.5089 1045.4535 1045.4535 837.4783 837.4783 885.4228 1094.5617 1142.5062 1142.5062 1271.5488 1223.6043 1271.5488 1324.6520 1372.5965 1372.5965 340.2111 340.2111 340.2111 439.2795 439.2795 439.2795 552.3636 552.3636 552.3636 683.4041 683.4041 731.3486 57.0215 57.0215 57.0215 283.1896 283.1896 283.1896 170.1055 170.1055 1170.1055 F F F G G G SeC C* C P P P E E E T T T P P P L L L G G G V V V L L L B M M I I I G G G 166.0863 166.0863 166.0863 807.3180 807.3180 855.2287 653.2491 653.2491 701.1545 396.1766 396.1766 396.1766 267.1298 267.1298 267.1298 710.2815 710.2815 758.1760 493.2352 493.2352 493.2352 1481.6613 1481.6613 1433.7168 938.3631 1255.4932 1207.5487 1255.4932 986.3192 986.3192 1368.5773 1368.5773 1320.6328 1150.5272 1198.4717 1198.4717 1051.2598 1099.4228 1099.4228 y7 y4 y2 y1 y5 y8 y7’ y9 y6’ y5’ y7 y6’ y7’ y5’ y2 x102 x102 2.4 1.6 807.31804 y7 y1 y4 Intensity, cps Intensity, cps 1.2 0.8 y5 y8 y12 733.05634 0 0 m/z 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 m/z 700 725 750 775 800 825 850

  4. 131.0405 131.0405 131.0405 131.0405 178.9857 178.9857 178.9852 441.0665 441.0665 489.0105 489.0105 489.0105 441.0665 393.1215 263.0893 310.0255 357.9704 262.0812 310.0255 310.0255 310.0255 528.0981 576.0425 576.0425 528.0981 576.0425 480.1535 528.0981 Figure S 2. MS-MS spectra and details of de novo sequencing of selenopeptides. x104 M M M M B B B M B B B M M M M B M M B M B S S S S S S S K K K K K K K 1,4 544.1716 496.2265 544.1716 592.1152 544.1716 496.2284 544.1713 234.1451 234.1449 234.1451 234.1451 234.1449 234.1451 234.1452 413.1325 365.1829 365.1829 413.1301 413.1307 365.1860 365.1855 147.1130 147.1131 147.1130 147.1130 147.1130 147.1131 147.1126 y2 Intensity, cps 0,7 y1 y3 y4 0 m/z 100 200 300 400 500 600 x104 1 y2 y2’ y2’’ y1 Intensity, cps y3 y2’’ 0,5 y4’ y4’’ b2 y4 y3’ y3’’ 0 m/z 100 200 300 400 500 600 y2 y2’ y2’’ y1 x103 8 y3 y3’ y4’ y4’’ y4 Intensity, cps 4 0 100 200 300 400 500 600 700 m/z

  5. x104 1 y3 Intensity, cps 0,5 y4 y1 y2 y5 y6 x103 0 m/z 5 100 200 300 400 500 600 700 800 900 Intensity, cps 2.5 x104 204.0899 204.0899 705.3269 705.3269 907.4045 955.3490 147.0684 147.0684 332.1849 332.1849 545.2962 545.2962 446.2278 446.2278 776.3642 776.3642 1 R R F F G G K K N N V V C C A A B M 175.1183 175.1191 878.4336 926.3781 684.2458 636.2913 425.1411 377.1963 983.3703 935.4249 750.3392 798.2741 537.2294 585.1718 354.1755 306.1577 0 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 m/z y4 Intensity, cps 0,5 y6 y1 y5 y3 y8 y2 y7 0 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 m/z x103 2,4 Intensity, cps y1 1,2 y4 y6 y3 y2 y8 y5 y7 0 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 m/z 216.0746 216.0746 686.1815 638.2374 129.0426 129.0426 363.1431 363.1431 581.2156 629.1601 542.1285 494.1835 773.2136 725.2691 E E S S F F B M S S G G S S R R 684.3134 732.2584 406.2034 406.2027 262.1512 262.1512 771.3455 819.2912 537.2441 585.1870 319.1725 319.1725 175.1192 175.1192 y4 y1 y5 y3 y6 y2 y7

  6. x102 5 Intensity, cps 2.5 y7 0 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 m/z y9’’ y10’ y6 y1 y5 y2 y9 y9’ y4 y8 y8’ y10 y10’’ x102 y3 2.6 x103 Intensity, cps 4 1.3 Intensity, cps 561.2911 561.2911 1079.4892 1127.4345 675.3341 675.3341 746.3712 746.3712 925.3562 877.4117 1008.4521 1056.3972 1224.4873 1176.5423 269.1823 156.1011 156.1011 432.2485 432.2485 y2 269.1823 y8 y10 y3 y7 R R 2 A A P P I I Y Y E E N N A A M B M M R R y4 y1 y5 0 y6 1243.5041 1195.5610 605.2911 653.2148 474.2419 474.2369 270.1820 270.1820 919.4025 967.3614 343.1984 343.2074 175.1193 175.1193 1082.4766 1130.4279 790.3750 837.3211 676.3329 724.3034 y9 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 m/z 0 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 m/z x102 5 Intensity, cps 2.5 y2 0 y9 y4 y5 y7 y8 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200 1250 1300 1350 1400 y1 y3 y6 m/z y10 1077.3932 1125.3371 1125.3371 1125.3371 310.0255 310.0255 262.0812 262.0814 698.1246 698.1246 698.1246 650.2227 909.2629 909.2629 909.2629 861.3184 1038.4432 1038.4432 1038.4432 990.3612 131.0405 131.0405 131.0405 178.9855 319.1088 367.0815 319.1025 367.0815 569.1246 569.1246 521.1948 569.1246 498.0874 450.1429 498.0874 498.0874 764.2656 812.2101 812.2101 812.2101 R R R R S S S S E E E E M B M M M B M M G G G G M M B M A A A A E E E E N N N N P P P P 175.1191 175.1191 175.1191 175.1191 262.1510 262.1510 262.1512 262.1510 933.4095 933.4095 933.4817 981.3542 391.1936 391.1936 391.1936 391.1936 1038.4432 990.4955 990.4787 990.4787 802.4511 802.45233 802.45233 802.45233 602.2893 602.3191 602.2893 602.2893 731.3963 731.3882 731.3882 731.3882 488.2464 488.2464 488.2464 488.2464 1121.5171 1169.5054 1121.5171 1121.5171 y9 y7 y1 y2 y6 y4 y5 y10 y8

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