Positional cloning of human disease genes a reversal of scientific priorities
This presentation is the property of its rightful owner.
Sponsored Links
1 / 15

positional cloning of human disease genes: a reversal of scientific priorities PowerPoint PPT Presentation


  • 124 Views
  • Uploaded on
  • Presentation posted in: General

positional cloning of human disease genes: a reversal of scientific priorities. Prior To 1989. disease phenotype. hypothesize function. clone the gene. chromosomal position. functional cloning. AFTER 1989. disease phenotype. chromosomal position. clone the gene. hypothesize function.

Download Presentation

positional cloning of human disease genes: a reversal of scientific priorities

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Positional cloning of human disease genes a reversal of scientific priorities

positional cloning of human diseasegenes: a reversal of scientific priorities

Prior To 1989

disease phenotype

hypothesize function

clone

the gene

chromosomal position

functional cloning

AFTER 1989

disease phenotype

chromosomal position

clone

the gene

hypothesize function

positional cloning

D Botstein, et al. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32: 314-331.

JM Rommens, …, LC Tsui, FS Collins. 1989. Identification of the cystic fibrosis gene: chromosome walking and jumping. Science 245: 1059-1065.

FS Collins. 1992. Positional cloning: let's not call it reverse anymore. Nat Genet 1: 3-6.


Botstein s insight on human genetics

Botstein’s insight on human genetics

cause-and-effect

gene

phenotype

positionally close

statistical correlation

marker

consider a mutagenesis and screening experiment that we might do in animals

genetic variations occur naturally and humans screen themselves for diseases

cause-and-effect is strongest when disease has Mendelian inheritance pattern

MARKER is any sequence that is variable and does not have to be in the gene

Q: why should there be a statistical correlation between the marker and gene?


Positional cloning of human disease genes a reversal of scientific priorities

genetic recombination is required tolocalize Mendelian disease genes in afamily based positional cloning study

  • w/o genetic recombination linkage across chromosome would be complete

    • approximately one recombination per chromosome per meiosis

    • human mutation rate is known to be 10-8 per site per generation

  • there is no benefit to genotyping more markers than genetic recombinations

  • size of family analyzed determines number of genetic recombinations

  • additional experiments needed to identify gene once localized to Mb region


Online mendelian inheritance in man currently lists 2993 phenotypes whose molecular basis is known

Online Mendelian Inheritance inMan currently lists 2993 phenotypeswhose molecular basis is known

the success of what came to be known as positional cloning was a tribute to an admission of ignorance; we did not know enough human biology to guess the likely gene for a disease so we focused instead on determining where the gene was on the chromosome; for the overwhelming majority of cases, the answer turned out to be a completely unknown gene that no scientist had hypothesized


Interesting traits tend to be less genetic and more environmental

interesting traits tend to be less genetic and more environmental

genetics is important

environment is important

Mendelian diseases

complex diseases

psychiatric disorders

human behaviour

interesting to scientists

interesting to the public


Making positional cloning work on diseases that the public cares about

making positional cloning work on diseases that the public cares about

despite the successes of positional cloning with Mendelian diseases analogous procedures for complex diseases FAILED spectacularly

failure was attributed to inability to get large enough families to compensate for weaker cause-and-effect in diseases that are not entirely genetic

sample sizes need not be a limitation if we do not restrict the studies to families and instead use affected individuals from the population

human population originated from “family” of 15,000 individuals that survived a near death experience 70,000 years ago (numbers controversial)

therefore, human variation occurs in haplotype blocks whereby polymorphisms are statistically correlated on length scales of a few kb’s

novel mutations complicate the situation but to a first approximation the way to find complex disease genes is to increase the number of makers


Snps haplotypes and tag snps

SNPs, haplotypes, and tag SNPs

(a) Chromosomal region with (three) sites of variation indicated. (b) A haplotype is a particular combination of SNP alleles along the chromosome. Here we show all 4 observed haplotypes in a surveyed population for a 6000 bp region with 20 SNPs. (c) Genotyping only 3 tag SNPs out of the available 20 is sufficient to distinguish between all observed haplotypes.

RA Gibbs, et al. 2003. The International HapMap Project. Nature 426: 789-796.


Positional cloning of human disease genes a reversal of scientific priorities

genome wide association study (or GWAS) to find complex disease genes in population based positional cloning

simplifying assumptions were made about the nature of disease variants

CDCV hypothesis: a few common allelic variants account for most of the genetic variance in disease susceptibility

Reich DE, Lander ES. 2001. On the allelic spectrum of human disease. Trends Genet 17: 502-510

CDRV hypothesis: a large number of rare allelic variants account for the genetic variance in disease susceptibility

Terwilliger JD, Weiss KM. 1998. Linkage disequilibrium mapping of complex disease: fantasy or reality? Curr Opin Biotechnol 9: 578-594

one common variant has more public health impact than many rare ones

GWAS is easy

GWAS is hard


Genetic loci found and heritability explained for several complex traits

genetic loci found and heritability explained for several complex traits

Manolio, et al. 2009. Nature 461: 747-753


Feasibility of detecting genetic variants by risk allele frequency and strength of genetic effect

feasibility of detecting genetic variants by risk allele frequency and strength of genetic effect


Pharmacogenomics genetic variation in response to treatment

pharmacogenomics: genetic variation in response to treatment

adverse drug reactions are a major cause of hospitalization and death; for USA 2.2 million serious cases and 100,000 deaths a year

human metabolism to detoxify drugs either makes them more water soluble for excretion in urine or more fat soluble for excretion in stool

variability associated with cytochrome P450 enzyme detoxification is 1000-fold; hence one person’s food is another person’s poison

BiDil is a patented (FDA in 2005) combination of two generic drugs specifically indicated for African Americans with congestive heart failure

Race in a Bottle (Scientific American August 2007)


19q13 13 il28b gene region containing genome wide determinant of response to hepc treatment

19q13.13 (IL28B gene) region containing genome wide determinant of response to hepC treatment

adapted from D Ge, et al. 2009. Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance. Nature 461: 399-401


Percent svr by genotype and c allele frequencies by population

percent SVR by genotype andC-allele frequencies by population

SVR (sustained virological response) refers to absence of detectable virus at end of follow up evaluation, indicating successful response to treatment

individuals homozygous for C-allele respond better to treatment (regardless of population) and C-allele is most often found in East Asian populations


An example of a flawed gwas study

an example of a flawed GWAS study

1 July 2010: this paper claimed to have identified genetic factors for longevity; it was published with much fanfare at Sciencexpress and got extensive media coverage; but within days the result was challenged by sharper eyes


And a lesson on the power of blogs which humbled that much hyped paper in just a matter of days

and a lesson on the power of blogswhich humbled that much-hyped paper in just a matter of days

7 July 2010: data of Sebastiani et al (right) are unusual in that all of the highest-ranked SNPs stand out by themselves and are not flanked by a column of highly-ranked SNPs as in other studies like the Wellcome Trust Case Control Consortium (left)

http://www.wired.com/wiredscience/2010/07/Serious-flaws-revealed-in-longevity-genes-study


  • Login