AVEQ - Clermont-Ferrand, 19-21 September2007
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AVEQ - Clermont-Ferrand, 19-21 September2007. WP9 Analysis for cold tolerance in oat. Institutes involved: Experimental Institute for Cereal Research,via S.Protaso 302, 29017 Fiorenzuola d’Arda (PC), Italy. IPGR, Sadovo, Bulgaria Suceava Genebank, Romania. Field trials: 2008 2009.

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WP9 Analysis for cold tolerance in oat

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Wp9 analysis for cold tolerance in oat

AVEQ - Clermont-Ferrand, 19-21 September2007

WP9

Analysis for cold tolerance in oat


Wp9 analysis for cold tolerance in oat

Institutes involved:

Experimental Institute for Cereal Research,via S.Protaso 302, 29017 Fiorenzuola d’Arda (PC), Italy.

IPGR, Sadovo, Bulgaria

Suceava Genebank, Romania


Wp9 analysis for cold tolerance in oat

Field trials:

2008

2009


Wp9 analysis for cold tolerance in oat

Number of cultivars to be evaluated in the field - 500


Wp9 analysis for cold tolerance in oat

Italian partner will screen for winter hardiness

winter accessions

Romanian partner will screen spring accessions for frost resistance

Bulgarian partner will screen winter and spring accessions for winter hardiness

Until end of the 2007 we need the information about growth habit (spring or winter types) from the coordinator of the multiplication WP2


Seeds

Seeds

  • Italian and Bulgarian partner need to receive the seeds until september 2008 for winter types

  • Romanian and Bulgarian partner need to receive the seeds until february 2008 for spring types.


Wp9 analysis for cold tolerance in oat

Plots of 2 m2

60 g (1000 seeds) for the samples

300 g. for standards


Protocols of field work

Protocols of field work

  • Each partner will register the meteo data;

  • The field injury will be visually estimated on a 0-9 scale according to Rizza et al. (1994).

  • After beginning the stable vegetable period the partner countries have to evaluate survival plants /1m2


Protocol for cold resistance

Protocol for cold resistance

  • Italian partner will receive all data from the Bulgarian partner and select 100 accession in order to make clorofile fluorescence evaluation test.

  • Romanian partner will make cold resistance tests (two regimes of - 100 C and - 120 C) and for comparing cold resistance of accessions it will be used formula NA .


Wp9 analysis for cold tolerance in oat

Chlorophyll fluorescence evaluation (Fv/Fm) on a set of cultivars (100?)

The standard treatment:

1 week-old plants were hardened at 3-1°C (day/night) 200mE m-2 s-1 for 4 weeks

and

subjected to a freezing treatment (–12°C)


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