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Mice diseases

Mice diseases. Bacterial diseases. MRM. Mycoplasma pulmonis is the cause of murine respiratory mycoplasmosis, or MRM In the respiratory tract, M. pulmonis preferentially colonizes the nasal passages and middle ears. Etiology - Mycoplasma pulmonis.

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Mice diseases

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  1. Mice diseases

  2. Bacterial diseases

  3. MRM • Mycoplasma pulmonisis the cause of murine respiratory mycoplasmosis, or MRM • In the respiratory tract, M. pulmonis preferentially colonizes the nasal passages and middle ears

  4. Etiology - Mycoplasma pulmonis • Most infections are subclinical- carried in nasopharynx • Disease induced by high cage ammonia or pathogens • Slow onset, chronic condition • Aerosol and direct contact • “Proximal Airway Disease”

  5. Mycoplasma pulmonis • Early signs: purulent nasal discharge, otitis media, sneezing, “sniffling”, porphyrin staining • Progresses to labored breathing, anorexia, lethargy, hunched posture • Bronchopneumonia

  6. Gross Lesions • In advanced disease, the lungs can have gray-purple patches that often have a "cobblestone" appearance. • Pictures…

  7. The left lobe of these lungs from a mouse with experimental MRM has a gray-purple "cobblestone" appearance due to accumulation of inflammatory cells in and around airways,

  8. Diagnosis • Commercial ELISA and IFA tests are useful for health monitoring, but serologic testing can be complicated by potential cross reactions with other rodent mycoplasmas.

  9. Treatment • Tetracycline limits losses • Enrofloxacin • Dosages p.58

  10. Tyzzer’s disease • Clostridium piliforme is the cause of Tyzzer’s disease. It is a gram-negative, motile, spore-forming bacillus. It has been grown in embryonated eggs and cell cultures

  11. Epizootiology • C. piliforme has a worldwide distribution and a wide host range, including rats, mice, gerbils, hamsters, guinea pigs, rabbits, dogs, cats, horses, nonhuman primates, and various wild species. Mice appear to be affected more often than rats.

  12. Clinical signs • Many affected rats and mice are simply found dead without prior signs being observed. If present, clinical signs include lethargy, ruffled fur, and diarrhea, as indicated by fecal soiling of the fur.

  13. Transmission • Transmission is presumably fecal-oral. • Possibilities include vertical transmission and introduction of spores via vermin*; contaminated feed; or incompletely sterilized food, bedding, or water. Morbidity and mortality are highly variable. *term applied to various animal species regarded as pests or nuisances and especially to those associated with the carrying of disease.

  14. The most consistent lesions in mice are multiple, pale, slightly depressed foci of necrosis in the liver, as seen here. Thickening and hyperemia of the intestine and pale areas in the myocardium also can be seen in some cases, but are not consistently present.

  15. Pathogenesis • C. piliforme becomes established in the intestine as a primary infection, usually in the ileum, cecum, or both. It spreads via the portal circulation to the liver, and from there via the blood to other organs, chiefly the heart.

  16. Diagnosis:  • Since the organism cannot be propagated on artificial media, histopathologic diagnoses are made by demonstration of the bacillus in the enterocytes, hepatocytes or cardiac myocytes  bordering necrotic foci in tissues stained with silver stains

  17. Treatment: • Oxytetracycline at 0.1 mg/ml water for 30 days was reported to abate mortality of an epizootic in mice.  Treatment is usually not warranted

  18. Salmonella • Salmonella enteritidis is a gram-negative bacillus. Salmonellae are identified according to serotype, of which over 1500 exist. • Virulence varies widely among serotypes; enteritidis and typhimurium are most commonly associated with disease in rodents

  19. Epizootiology • Salmonellosis was a major epizootic and enzootic disease of laboratory mice prior to the 1950s, but clinical salmonellosis in mice is now rare. Transmission is fecal-oral. Salmonellae in general are not very host specific, and carriers are common among exposed populations. Therefore, potential sources of infection for laboratory mice include vermin; contaminated food, water, bedding, and fomites

  20. Clinical signs • Most infections are subclinical. • Signs in mice infected with weakly virulent serotypes vary from none to occasional losses among sucklings and weanlings

  21. Gross lesions • This mouse with salmonellosis has pale foci of necrotizing and suppurative hepatitis at the margins of the liver lobes (arrows). The spleen is greatly enlarged due to production of increased numbers of neutrophils, or myeloid hyperplasia.

  22. Diagnosis • Diagnosis of salmonellosis is by culture and serotyping. Several sites should be cultured, including liver, spleen, mesenteric lymph nodes, blood, and intestinal contents.

  23. Control • Special attention should be given to vermin control and procurement and storage of bedding materials and food, which are easily contaminated by wild rodents. If salmonellosis is diagnosed in a research facility, the facility should be immediately quarantined, the affected populations destroyed and their environment decontaminated. • ZOONOTIC disease!!!

  24. Skin • Staphylococcus aureus is the major pathogen for the skin of mice and rats. • Staphyloccus aureus is a gram-positive coccus that typically grows in clusters. It commonly inhabits the skin, skin glands, nasopharynx, and intestine of many host species.

  25. Epizootiology • Staph. aureus is a common commensal of many species. It also is common in the environment. • Human carriers can be an important source of infection for rodent colonies

  26. Staph. aureus is associated mostly with dermatitis in the form of pyoderma or ulcerative dermatitis. The face, shoulders, neck, and ears are most commonly affected.

  27. Staphylococcus aureus • Abscesses and granulomas, most commonly affecting the face and tissues around the base of the tail, also occur and can be associated with fight wounds

  28. Staphylococcus aureus • The best methods of control are improved sanitation, frequent sanitizing of cages and other equipment, and elimination of equipment that could cause skin injury • Topical treatment with Nolvasan BID

  29. Viral diseases

  30. SDAV (Sendai Virus) SV • Sialodacryoadenitis virus (SDAV), another coronavirus (Parainfluenza 1), is one of the most common viruses found in laboratory rats and mice. It is highly contagious, and is spread by direct contact with infected animals or by respiratory aerosol

  31. SDAV • The incubation period for SDAV less than 1 week. In naive populations, a sudden high incidence of overt disease with sneezing, porphyrin-stained nasal and ocular discharges (as seen in this image), cervical edema, corneal ulceration, and kerato­conus may be the first indications of a problem

  32. Sendai Virus • This image shows swollen submandibular salivary glands (arrows) in a mouse with SDAV. SDAV has tissue tropism for the submaxillary and parotid salivary, exorbital, Harderian, and intraorbital lacrimal glands

  33. In rats and mice, few gross morphologic lesions are seen in uncomplicated Sendai infections. The lungs can be focally reddened and atelectatic with serous fluid visible in the pleural and pericardial cavities.

  34. Mouse Hepatitis Virus (MHV) • Mouse hepatitis virus (MHV) and sialodacryoadenitis virus (SDAV) are frequently encountered coronaviruses of mice and rats respectively • Some primarily infect the gastrointestinal system; some the respiratory tract; some the brain

  35. MHV • Mice (Mus musculus) are the only natural hosts. MHV is extremely contagious with prevalence rates exceeding 80% in outbreaks. Active infection lasts 2-3 weeks, during which mice shed the virus in gastrointestinal and respiratory excretions. Direct contact with shedding mice, contaminated cell lines, fomites, or airborne particles are the important routes of viral transmission.

  36. MHV • An epizootic can produce nonspecific clinical signs in naïve*, juvenile mice, such as runting, as shown here, or failure to thrive not previously subjected to experimentation or a particular experimental situation <made the test with naive mouse>

  37. MHV • This image shows large coalescing cream-colored friable foci (arrow) of necrosis that result when acute multifocal hepatitis progresses to chronic active hepatitis in nude mice. Gross pathology in immune-incompetent mice is more generalized and pro­gressive than in immune-competent mice.

  38. Mouse Hepatitis Virus • Enterotropic versus respiratory strains • Extremely contagious by many routes • Nursing pups: diarrhea and mortality • Weanlings: Obstipation • Adults: Hunched posture, weight loss, rough hair coat, variable mortality • No latent infections- stop breeding • ELISA

  39. Mouse Hepatitis Virus • Diagnosis of latent infections is dependent on the histologic demonstration of large, multinucleate syncytial cells (arrow) in the liver, brain, or mucosal epithelium of the intestine

  40. Rotavirus • Rotavirus, another genus of the family Reoviridae, is associated with clinical disease. Rotaviruses affecting mice and rats respectively are mouse rotavirus, a group-A rotavirus associated with the syndrome, epizootic diarrhea of infant mice (EDIM)

  41. Rotavirus • Epizootic Diarrhea of Infant Mice (EDIM) • Most susceptible: birth to 17 days of age • Fecal-oral transmission • Yellow, watery diarrhea in 14-17 day old pups • Death with a full stomach (versus reovirus), shortened intestinal villi • Unapparent viral carriers • ELISA

  42. EDIM • Neonatal diarrhea is the most prominent sign. Watery yellow stool accumulates around the anus and tailbase, soiling the coats of neonatal pups and their dams. Pups appear stunted and lethargic and have distended abdomens. Mortality rates are low

  43. Rotavirus

  44. Parasites

  45. Myobia musculi, Radfordia affinis, and Myocoptes musculinus • Common • Transmitted by direct contact • May be subclinical, or develop pruritus, scruffy coat, patchy alopecia, self-trauma, pyoderma • Typically found on back and head • Mist with ivermectin (0.1%) for 3 weekly treatments

  46. Myobia musculi • Myobia musculi lesions in a pet white mouse, characteristic of hypersensitivity to the mites. Intense pruritus, often directed at the neck and ears, leads to self-mutilation. Early lesions consist of subtle hair thinning on the dorsal neck and shoulders • Acariasis???

  47. Myobia musculi Single claw

  48. Radfordia affinis Two claws

  49. Myocoptes musculinus

  50. Pinworms • Common • Fecal-oral transmission • Usually subclinical, but may cause rectal prolapse • Fecal floatation (Aspiculuris tetraptera) or tape test (Syphacia obvelata) • Ivermectin misting (others more labor intensive) • Clean environment well

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