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Tetraphenylphosphonium Bromide TTP

Tetraphenylphosphonium Bromide TTP. Large Molecule-Small Molecule Interaction Program. SHEA HIGH SCHOOL July 13-17, 2009 Ms. La Roche Cesar Luzon, Janice Miranda, Maldine Santos, Estefania Tabares. Tetraphenylphosphonium Bromide TPP. Formula : CH 3 P(C 6 H 5 ) 4

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Tetraphenylphosphonium Bromide TTP

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  1. Tetraphenylphosphonium Bromide TTP Large Molecule-Small Molecule Interaction Program. SHEA HIGH SCHOOL July 13-17, 2009 Ms. La Roche Cesar Luzon, Janice Miranda, Maldine Santos, Estefania Tabares.

  2. Tetraphenylphosphonium Bromide TPP. Formula: CH3P(C6H5)4 Mol WT: 419.30 Synonyms: Bromure de tétraphénylphosphonium; Bromuro de tetrafenilfosfonio; Classification: ORGANOPHOSPHINES. Physical state: white to off-white crystalline powder. Melting Point:295 - 299 C Solubility in water: Soluble. Stability: Stable under ordinary conditions.

  3. Electrophoresis

  4. Observations: both the linear and the uncut plasmid with TPP travelled 1mm further showing the small molecule bonded with the DNA molecule.

  5. Cary Spectrophotometer

  6. Salmon DNA +TPP

  7. HYPERCHEM MAJOR GROOVE MINOR GROOVE INTERCALATING

  8. Smart spec.

  9. DISCUSSION: • Hyperchem analyses led to the prediction that tetraphenylphosphonium Bromide would bond to DNA. The experimental data shows a shift in the melting curve when TPP is added, agreeing with this prediction • No significant changes were made in the experimental procedures. • Light banding in electrophoresis gels suggest using a larger amount of the • Samples. Practice pipetting into wells is suggested. • Repeated trials of both experimental procedures are recommended for further research. • The melting point of Salmon DNA was 70⁰C on the Smart Spec and 59⁰C on the Cary spectrophotometer. • The difference in the melting point could be due to the fluctuating temperatures with the Smart Spec Plus. • The melting point of the Salmon DNA with the small molecule TPP: Cary - appears to be 49⁰C. There are three other indications of melting in different phases. • Smart Spec – points to two melting points, 68⁰C and 83⁰C.

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