High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules
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High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules Dawn A. Bonnell, University of Pennsylvania, DMR 0425780. Some motions of myosin V are too fast to resolve by polTIRF. Tilting Angle (  ) and Rotational Diffusion (  ) of Myosin V Lever Arm. 90 . . 45 .

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High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules

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High speed fluorescence polarization measurements of single myosin v molecules

High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules

Dawn A. Bonnell, University of Pennsylvania, DMR 0425780

Some motions of myosin V are too fast to resolve by polTIRF

Tilting Angle () and Rotational

Diffusion () of Myosin V Lever Arm

90

45

4 ms

0

90

4 ms

45

0

sec

0

0.4

0.8

1.2

AR Dunn & JA Spudich, NSMB, 2007

  • Time resolution increased 100x from 40 to 4 ms.

  • Researchers at the Nanoscale Science and Engineering Center (NSEC) at the University of Pennsylvania want to visualize novel myosin V motions between steps. Understanding Nature’s molecular motors may explain certain diseases and inspire man-made nano-machines.


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