PACE 2C. Genetic probe screening for C. Trachomatis & N. Gonorrhoeae in Endocervical and Male urethral specimens. Heidi Philips and Michael S. Joseph. Outline. Biological background on Chlamydia Trachomatis and Neisseria Gonorrhoeae Flow chart Biology of the PACE 2C Assay Applications
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Genetic probe screening for C. Trachomatis & N. Gonorrhoeae in Endocervical and Male urethral specimens
Heidi Philips and Michael S. Joseph
One swab, one tube, two test convince.
- Samples must be obtained using the PACE Specimen Collection Kits.
- All reagents aside from the probe reagent must reach room temperature prior to use.
-The solid phase must be reconstituted in the selection reagent prior to use.
- Sample must reach room temperature prior to use.
- Chemiluminescent probes
- Leader Illuminometer
- Precise hybridization conditions
- Lyophilized probe
The use of single-strand DNA probes with chemiluminescent labels that are complementary to the ribosomal RNA of the target organism allows the DNA probes to combine with the rRNA of the target organism to form stable DNA:RNA hybrids.
Targeting of rRNA
-Separation- Forceful washing: Angle wash to front sides of tube with the appearance of foam head to ensure adequate wash procedure.Sensitivity
The Negative Reference and Positive Controls provided, control for the PACE 2C assay ONLY.
C. trachomatis N. gonorrhoeae
Sample Positive Control Positive Control
Number of Replicates 69 69
Mean Response (RLU) 1837 2165
Standard Deviation (RLU) 329 425
Coefficient of Variance 5.6% 5.1%
- Temperature Dependent
- Uniform equilibration of reaction tubes needed
-Separation- Forceful washing: Angle wash to front sides of tube with the appearance of foam head to ensure adequate wash procedure.