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ChIP-seq Analysis of TBX2, TBX4, and RUNX3 Interactions in Hs68 and BF Cells

This study presents a comprehensive ChIP-seq analysis of TBX2, TBX4, and RUNX3 in Hs68 and BF cells, focusing on their interactions with various control factors such as CBX4, CBX6, and RING1. We utilized specific primers and examined input percentages to quantify binding affinities and regulatory impacts. The results reveal intricate epigenetic relationships and transcriptional regulation mechanisms that play crucial roles in cellular functions and developmental processes. The data presented here can further our understanding of gene regulation in human cells.

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ChIP-seq Analysis of TBX2, TBX4, and RUNX3 Interactions in Hs68 and BF Cells

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  1. Additional file 4: Figure S3 A. TBX2 0.15 0.20 HPH1 CBX4 Control Control 0.15 CBX7 ChIP-seq 0.10 % input % input 0.10 0.05 0.05 TBX2 0 0 0.3 CBX6 1.5 HPH2 Control 0.2 Control 1.0 % input Primers: % input 1 2 3 3a 4 0.5 0.1 0 Hs68 cells BF cells 0 0.3 CBX7 0.8 RING1 Control Control 0.2 0.6 % input % input 0.4 0.1 0.2 0 0 0.8 1.5 0.8 CBX8 0.6 MEL18 RING2 Control 0.6 Not detected 1.0 Control Control 0.4 % input 0.4 % input % input 0.2 0.5 0.2 0 0 0 PS1 PS2 PS3 PS3a PS4 PS1 PS1 PS2 PS2 PS3 PS3 PS3a PS3a PS4 PS4

  2. B. TBX4 0.2 HPH1 0.10 0.20 0.6 HPH1 CBX4 CBX4 Control Control Control Control 0.15 0.4 % input 0.1 % input % input % input 0.05 0.10 CBX7 ChIP-seq 0.2 0.05 0 0 0 0 0.15 HPH2 CBX6 CBX6 1.5 2.0 0.6 HPH2 TBX4 Control Control Control 1.5 0.10 Control 1.0 0.4 % input % input % input 1.0 % input 0.5 0.05 0.5 0.2 0 0 Primers: 1 2 2a 3 4 0 0 0.6 0.15 0.20 1.0 CBX7 CBX7 RING1 HPH3 Hs68 cells BF cells Control Control Control 0.4 0.15 Control 0.10 % input % input % input 0.10 % input 0.5 0.2 0.05 0.05 0 0 0 0 2.0 RING1 1.5 0.8 0.6 Control 1.5 MEL18 MEL18 RING2 0.6 1.0 Control Control 0.4 Control % input 1.0 0.4 % input % input % input 0.5 0.5 0.2 0.2 0 0 0 0 PS1 PS1 PS2 PS2 PS2a PS3 PS3 PS3a PS4 PS4 PS3 PS1 PS2 PS2a PS4 2.0 RING2 1.5 Control 1.0 % input 0.5 0 PS1 PS2 PS2a PS3 PS4

  3. C. RUNX3 CBX7 ChIP-seq RUNX3 1 2 5 4 3 5b 6 7 8 Primer sets: BF cells Hs68 cells 6 5 4 CBX6 3 % input Control 2 1 0 1.2 CBX6 0.8 Control % input 0.4 0 4 CBX8 3 % input Control 2 1 0 10 8 RING1 6 Control % input 4 2 0 5 4 RING2 Control 3 % input 2 1 0 0.8 0.6 H3K27me3 Control % input 0.4 0.2 0 0.15 0.10 H3K4me3 % input Control 0.05 0 PS2 PS4 PS3 PS5b PS6 PS2 PS4 PS3 PS5b PS6

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