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Gene Expression in Arabidopsis thaliana Rosette Leaves Treated with Paraquat

Study on Arabidopsis thaliana gene expression in response to paraquat treatment, analyzing changes in nuclear gene expression. Results indicate activation of distinct gene sets different from those induced by singlet oxygen.

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Gene Expression in Arabidopsis thaliana Rosette Leaves Treated with Paraquat

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  1. Expression data from Arabidopsis thaliana (Ler) rosette leaves treated with paraquat (methyl viologen) Izuddin Abu and Rayan Alansari Leaves harvested 1, 2& 4 h after spraying either with 20 µM paraquatin 0.1% Tween or with Tween alone for RNA extraction and hybridization on Affymetrix ATH1 microarrays. Plants grown on soil for 3 weeks under continuous light at 90 mmol. m-2 . s-1. cDNA were prepared from total RNAs from two separate biological experiments and subsequent synthesis of biotin-labeled complementary RNA recommended by Affymetrix. Microarray is used to detail Arabidopsis gene expression in response to paraquat which may enhance generation of superoxide and hydrogen peroxide inside plastids. Within a few hours after paraquat treatment changes in nuclear gene expression occur. Distinct sets of genes were activated that were different from those induced by another reactive oxygen species, singlet oxygen.

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