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Analysis of Responder and Transactivator Genes in Transgenic Mice

Comparative real-time PCR analysis of responder (GFP) and transactivator (α-MHC-tTA) genes in transgenic mice versus controls. No correlation between GFP and α-MHC-tTA expression observed.

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Analysis of Responder and Transactivator Genes in Transgenic Mice

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  1. Supplemental Figure 3 Relative expression Relative expression Supplemental Figure 3. ComparativeReal time-PCR analysis of the responder (GFP, upper panel) and transactivator (α-MHC-tTA, lower panel) genes in 7 days-old transgenic/control mice. Samples 1-7 are bitransgenic mice, while c1-c2 are controls carrying the solely α-MHC-tTA and c3-c6 are WT. There is no correlation between the expression of GFP and α-MHC-tTA. The cut-off (red line) of the expression in the GFP assay is 6.

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