AQUAGARD-T

1. SUNGIL PHARMA A concern of A effervescent tablet disinfectant AQUAGARD-T Good Effervescent ! Long Lasting ! High Effectiveness ! Very Convenient ! Varied Application !

2. Features of Aquagard-T • Good Effervescent ! • Long Lasting ! • High Effectiveness ! • Very Convenient ! • Varied Application !

3. Aquagard-T… • Aquagard-T is a effervescent tablet disinfectant. • Aquagard-T is a very powerful disinfectant containing sodium dichloroisocyanurate (NaDCC) widely applied for the sterilization of drinking water, or fighting against infectious diseases, or act as disinfectant in raising livestock, poultry and fish.

4. Aquagard-T… • Aquagard-T is an oxidising compound which releases chlorine to produce hypochlorous acid and hypochlorite ion in the presence of water. It is generally considered that the lethal action on organism is due to chlorination of cell protein or enzyme systems by nonionised hypochlorous acid, causing hydrolysis of peptidic chains of cellular membranes of pathogenic germs. Chlorine-releasing substances are bactericidal to most gram-positive and gram-negative bacteria, bacterial spores and viruses.

5. NaDCC(Sodium Dichloroisocyanurate) (Structural formula of NaDCC)

6. Hypochlorous acid + 2HOCl germicidal activity H+ + OCl- Hypochlorite ion NaDCC(Sodium Dichloroisocyanurate) + 2H2O (Hydrolysis of NaDCC)

7. pH of NaOCl solutions pH of Aquagard-T solutions Aquagard-T : NaDCC(Sodium Dichloroisocyanurate) • The germicidal activity of hypochlorous acid is 80 times higher than hypochlorite ion. • The presence of HOCl in solution of chlorine containing compound

8. Inorganic compoundSodium hypochlorite (NaOCl) Calcium hypochlorite (CaOCl) Chloline dioxide (ClO2) Chlorine containing compound • Chlorine used widely as a disinfectant and bleaching agent unstable, less germicidal activity • Organic compound Sodium dichloroisocyanurate (NaDCC) very stable, predominantly germicidal activity

9. Features of Aquagard-T • Good effervescent high technique for making effervescent tablets • Long lasting free available chlorine is present for 5days in Aquagard-T solutions • High effectiveness broad spectrum disinfectant (active to spore forming bacteria, naked virus) • Very convenient easy to handle and safe • Varied application used widely for disinfection of poultry, pig, cattle and sheep, livestock houses and facilities, drinking water

10. Weak Disinfectants Intermediate Disinfectants Strong Disinfectants surfactant, quaternary ammonium chloride cresol, phenol, ortho, alcohol chlorine, iodine, Aldehydes, ethylene oxide Microbiocidal activity of various disinfectants Very Sensitive Sensitive Resistant Very Resistant Septic bacteria (Pasteurella sp) Enveloped virus (HC, TGE, ILT, IB, ND) Non-enveloped virus (Enterovirus, Adenovirus, FMD, IBD, Reo) Spore forming bacteria (B. anthracis, Clostridium)

11. Microbiocidal activity of Aquagard-T * Total available chlorine

12. Bactericidal activity of Aquagard-T Phenol coefficiency of Aquagard-T against bacteria

13. Bactericidal activity of Aquagard-T Effectiveness of Aquagard-T against spore forming bacteria Effective dilution rate, ( ) : Exposure time

14. Bactericidal activity of Aquagard-T The sporicidal activity of disinfectants

15. Virucidal activity of Aquagard-T Virucidal activity against poultry viruses ○ : inactivated, × : not inactivated

16. Virucidal activity of Aquagard-T Virucidal activity against pig viruses ○ : inactivated, × : not inactivated

17. Fungicidal activity of Aquagard-T

18. The efficacy test scope Total bacterial colony counts on foot bath in entrance of farm Unit : CFU/ml, - : <1×101CFU/ml

19. Method : According to regulation of NVRQS Experiment : Research Institute for Veterinary Science in Konkuk Univ. Results : Bactericidal activity

20. Method : According to regulation of NVRQS Experiment : Research Institute for Veterinary Science in GyeongsangNational Univ. and Cheonbuk National Univ. Results : Virucidal activity

21. Composition of Aquagard-T • Each tablet (13g) contains 5g of Sodium dichloroisocyanurate (= 3g of available chlorine) and Component A (effervescent agent) Component B (pH controlling agent)

22. Application of Aquagard-T • Disinfection of virus and bacteria in livestock housing, farms (floor, wall), facilities and equipment, the animal body • Drinking water treatment • Teat dip or spray (mastitis prevention) • Disinfection of pathogen • General bacteria (Salmonella spp.), Brucella spp., Streptococcus spp. and so on. • Foot and Mouth Disease virus, Hog Cholera virus, PRRS virus, Newcastle Disease virus, Avian Influenza Virus, Avian Pneumovirus, Inclusion Body Hepatitis virus and so on

23. Use of Aquagard-T(Bacteria) * Dilution rate is the most effective dilution rate of results of determination of the efficacy of disinfectant against general bacteria and specific pathogen.

24. Use of Aquagard-T(Virus) * Dilution rate is the most effective dilution rate of results of determination of the efficacy of disinfectant against general bacteria and specific pathogen.

25. Use of Aquagard-T(Water Treatment) • Dissolve Aquagard-T in water tank every other day or every day if necessary. • Dilute 1 tablet per 500 ~ 750 L of drinking water according to water quality and farm condition.

26. Use program for poultry • Poultry houses and facilities - Thorough cleaning with water - Spray Aquagard-T solution not less than 2 times before placement of chicks. - Spray Aquagard-T solution during growing period if necessary.

27. Use program for poultry • Foot bath - For disinfect boots by dipping before entering each house, replace the Aquagard-T solutions every 5 days or sooner when soiled by heavy use. • Transport - For prevention of disease transmission through trucks and crates by disinfecting them with Aquagard-T. - Use in wheel baths and renew every 5 days or according to volume of traffic.

28. Use program for dairy • Dairy houses and facilities - Thorough cleaning with water - Spray Aquagard-T solution, eliminate all bacteria, viruses and fungi.

29. Use program for dairy to prevent Mastitis • Preparation of disinfection • Dissolve 1 Aquagard-T tablet per 1L of water and move to dipping cup or sprayer for disinfection. • Prior to milking - Clean milk starts with clean teats. - Teat and udder spraying will remove all bacteria, viruses and fungi. • After milking - Teat dip or spraying will decontaminate the teats and prevent mastitis

30. Use program for Milking Dipping method Spray method 2) Removal of disinfectant - With clean and disinfected towel 1) Disinfection prior to milking - Removal of contaminant on teat 3) Prior to milking - More than 5 times 4) Massage and final cleaning - With clean and disinfected towel 5) Attaching the milking mashing - Vacuum-blocking 6) Disinfection after milking - Prevention of teat canal

31. Package of Aquagard-T • 10 tablets / plastic container • 100 tablets (1.3kg) / plastic container • 2,000 tablets / carton

32. Storage and shelf-life of Aquagard-T • Storage Keep under cool and dry conditions and protect from light • Shelf-life 24 months after manufacturing

33. Efficacy Test Report

34. Effect of Aquagard-T on E. Coli in Water

35. Precautions of Aquagard-T • Keep out of reach of children. • Do not dissolve in strong alkali (over pH 10) or acidic (below pH 3) solution. • Do not exceed the recommended dosage. • For 5 days, from before 2 days to after 2 days of vaccination, do not disinfect drinking water. • Do not place near fire or strong inflammables. • Do not place in metallic vessel more than 2 hours. • In case of drink diluted solution, immediately seek for medical advice.

36. SUNGIL PHARMA A concern of Thank You