1 / 40

Supplemental Figure S1

Supplemental Figure S1.

yael
Download Presentation

Supplemental Figure S1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Supplemental Figure S1 Supplemental Figure S1. MALDI-TOF/TOF MS analysis of mouse serum glycans. MS/MS spectra were annotated using GlycoWorkbench software which calculates the percentage of experimental fragments that match with the theoretical fragments generated in silico. For each m/z value, the shown structure is the one which presented the largest number of fragments that match with the theoretical fragments. Only the B-, C-, Y- and Z-type ions are shown. The B- and C-type ions resulting from terminal reductive fragmentation are not systematically illustrated by the software. Most of the observed ions were sodiated. However, the sodiated ion with m/z 428 (NeuGc) has given rise to the ion with m/z 406, which is a desodiated and protonated form of the ion with m/z 428, and to the ion with m/z 374, which is a decarboxylated form of the ion with m/z 406. The ions with m/z 374 and 406 were frequently observed on the MS/MS spectra.

  2. 1580 Parent ion with m/z

  3. 1784 Parent ion with m/z *877 d1389 *953 d1795 d472 *428 *1807 * The methodology for the extraction of N-glycans can introduce free O-glycans from serum. Therefore, ions 428, 877 and 953 likely are fragments of the parent ion 1807 that matches with a O-glycan ( ). d Ions 472 and 1389 are fragments of the parent ion 1795.

  4. 1795 Parent ion with m/z d1784 d1506 *877 d1565 *1807 *428 * Ions 428 and 877 are fragments of the parent ion 1807. d Ions 1506 and 1565 are fragments of the parent ion 1784.

  5. 1836 Parent ion with m/z *953 *877 *1807 * Ions 877 and 953 are fragments of the parent ion 1807 wich is likely a O-Glycan (see parent ion 1784). Remark: more fragments match with a structure showing a fucose on the terminal GlcNAc rather than a structure with a fucose on the reductive terminal GlcNAc.

  6. 1988 Parent ion with m/z *877 d1999 d1593 *1606 *2012 *472 *406 d1576 *1795 *374 * Ions 374, 406, 472, 877, 1606 and 1795 are fragments of the parent ion 2012. d Ions 1576 and 1593 are fragments of the parent ion 1999.

  7. 1999 Parent ion with m/z *877 *1606 *2012 * Ions 877 and 1606 are fragments of the parent ion 2012

  8. 2012 Parent ion with m/z *1592 *1999 * Ion 1592 is a fragment of ion 1999.

  9. 2040 Parent ion with m/z *1157 *2012 *877 *428 * Ions 428, 877 and 1157 are fragments of the parent ion 2012

  10. 2192 Parent ion with m/z *877 *1780 *428 *2186 *1998 *406 *472 *374 * 374, 406, 428, 472, 877, 1780 and 1998 are fragments of the parent ion 2186, a monoantennary fucosylated and sialylated (NeuGc) glycan.

  11. 2216 Parent ion with m/z *1781 *2244 406 *1837 *1317 374 * 1317, 1781 and 1837 are fragments of the parent ion 2244.

  12. 2244 Parent ion with m/z *1999 *877 *1852 d1361 *1980 *406 *2257 *374 *650 * 374, 406, 650, 877, 1852, 1980 and 1999 are fragments of the parent ion 2257. d 1361 can be attributed to the protonated Z-type ion that comes from ion 2257 (see parent ion with m/z 2257).

  13. 2257 Parent ion with m/z *2244 *1837 d1361 406 374 * Ion 1837 is a fragment of the parent ion 2244. d Ion 1361 can be attributed to the protonated Z-type ion:

  14. 2396 Parent ion with m/z *877 *1565 *2014 *428 *472 *1995 *1592 *1984 *406 *2420 *374 *2144 d 1268 * Ions 374, 406, 428, 472, 877, 1565,1592, 1984, 1995, 2014,2144 are fragments of the parent ion 2420. d Ion 1268 was not able to be attributed.

  15. 2420 Parent ion with m/z BY CZ *2042 *2448 406 374 * Ion 2042 is a fragment of the parent ion 2448.

  16. 2448 Parent ion with m/z *2461 *877 *650 * Ions 650 and 877 can be attributed to ion 2461. Remark: more fragments matched with a structure displaying a fucose on antennae with Gal-Gal motif.

  17. 2461 Parent ion with m/z *2042 *2448 406 374 * Ion 2042 is a fragment of the parent ion 2448.

  18. 2607 Parent ion with m/z *374 *877 *406 d 855 *2173 *472 *2230 *1317 *1081 *2635 * Ions 374, 406, 472, 855, 877, 1081, 1317, 2173 and 2230 are fragments of the parent ion 2635. d Ion 855 is a protonated and desodiated form of the ion 877. Remark: More ions match with a structure showing a substituted LacNAc antennae instead of a polysialylated structure.

  19. 2635 Parent ion with m/z *406 *374 d1752 d196 *855 d1998 * Ions 374, 406 and 855 are fragments of the parent ion 2635. d Ions 196, 1752 and 1998 were not able to be attributed to a parent ion.

  20. 2796 Parent ion with m/z d1998 *877 *406 *374 *1983 *2433 *428 d2448 *855 § 196 *2809 d1143 d2822 *1720 d1593 § 819 *650 * Ions 374, 406, 650, 855, 877, 1720, 1983 and 2433 are fragments of the parent ion 2809. d Ions 1143, 1593, 1998 and 2448 are fragments of the parent ion 2822. § Ions196 and 819 were not able to be attributed to a parent ion.

  21. 2809 Parent ion with m/z *1998 *2448 *2244 *1143 d 406 *2822 *1592 d 374 *1793 *939 * Ions 939, 1143, 1592, 1793, 2244 and 2448 are fragments of the parent ion 2822. d Ions 374 and 406 are fragments of the parent ion 2809.

  22. 2822 Parent ion with m/z 2837 2852

  23. 2837 Parent ion with m/z *1998 *2447 *877 *2852 *2576 *1081 *428 *1793 *1143 *1592 *2242 * Ions 428, 877, 1081, 1143, 1592, 1793, 1998, 2242, 2447 and 2576 are fragments of the parent ion 2852.

  24. Parent ion with m/z 2852

  25. 2867 Parent ion with m/z *2852 *1997 *877 *2447 *1143 * Ions 877, 1143, 1997 and 2447 are fragments of the parent ion 2852.

  26. 2895 Parent ion with m/z 2852 *2910 *2505 *2056 *877 *2448 *939 * Ions 877, 939, 2056, 2448 and 2505 are fragments of the parent ion 2910.

  27. 2910 Parent ion with m/z

  28. Parent ion with m/z 3026 406 374

  29. 3056 Parent ion with m/z *3026 *2173 *2622 406 d 855 *1967 374 * Ions 1967, 2173 and 2622 are fragments of the parent ion 3026. d Ion 855 is a protonated and desodiated form of ion 877.

  30. 3228 Parent ion with m/z *2839 *650 *406 *1998 *1268 *877 *374 *3244 * Ions 374, 406, 650, 877, 1268, 1998 and 2839 are fragments of the parent ion 3244.

  31. 3244 Parent ion with m/z

  32. 3259 Parent ion with m/z *3244 *2839 *1268 *1997 d 406 d 374 *1793 * Ions 1268, 1793, 1997 and 2839 are fragments of the parent ion 3244. d Ions 374 and 406 are fragments of the parent ion 3259.

  33. 3288 Parent ion with m/z *2839 *3244 d 406 *1997 d 374 *1268 * Ions 1268, 1997 and 2839 are fragments of the parent ion 3244. d Ions 374 and 406 are fragments of the parent ion 3288.

  34. 3302 Parent ion with m/z *3244 *2839 *1997 *1268 * Ions 1268, 1997 and 2839 are fragments of the parent ion 3244.

  35. 3635 Parent ion with m/z *2839 *877 *650 *428 *406 *374 *3663 * Ions 374, 406, 428, 650, 877 and 2839 are fragments of the parent ion 3663.

  36. 3663 Parent ion with m/z 1 2 * 2839 d 406 * 3288 d 374 Two potential structures (1 and 2) were observed, but more fragments matched with the structure 1 than the structure 2. * Ions 2839 and 3288 are fragments of the parent ion n°2. d Ions 374 and 406 are related to NeuGc sialic acid

  37. 3678 Parent ion with m/z *2839 *3288 *3693 d 406 *1983 *1268 * Ions 1268, 1983, 2839 and 3288 are fragments of the parent ion 3693. d Ion 406 is related to NeuGc sialic acid.

  38. 3693 Parent ion with m/z 406

  39. 3708 Parent ion with m/z *3693 *3288 406 *1268 *1984 374 *2433 * Ions 1268, 1984, 2433 and 3288 are fragments of the parent ion 3693.

  40. 4084 Parent ion with m/z *3230 *4128 *2837 *406 * Ions 374, 406, 650, 877, 2837, 3230 are fragments of the parent ion 4128 ( ) *877 *650 *374

More Related