1 / 35

The Role of Restriction Endonucleases in Genetic Engineering and Biotechnology

This overview explores the significance of restriction endonucleases, enzymes that cut DNA at specific sites, in the field of genetic technology. Originating from bacterial defense mechanisms against viruses, these enzymes are pivotal in creating recombinant DNA. Key applications include genetic recombination for GMOs, DNA fingerprinting, and polymerase chain reactions (PCR). Learn about their role in pivotal discoveries, such as the first recombinant DNA creation by Boyer and Cohen, and their use in modern genetic research, including microarrays and genome-wide association studies.

xannon
Download Presentation

The Role of Restriction Endonucleases in Genetic Engineering and Biotechnology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. GENETIC TECHNOLOGY HONORS BIOLOGY 2A Motzko

  2. Constantine Fahlberg - Saccharin (1879)

  3. James Schlatter - Aspartame (1965)

  4. Percy Spencer -Microwave Oven (1945)

  5. John Harvey Kellogg - Corn Flakes (1899)

  6. Alexander Fleming - Penicillin (1927)

  7. Werner Arber(1969)1st To Isolate Restriction Endonucleases (Enzymes)

  8. Restriction Endonucleases

  9. Restriction Endonucleases • Enzymes that cut DNA at specific locations on the strand known as restriction sites • Naturally produced by bacteria to protect them against viruses • Restriction sites differ depending upon the sequence of nucleotides • When restriction enzymes cut the DNA strand they can leave either “blunt ends” or unpaired nucleotides called “sticky ends”

  10. Restriction Enzymes digest/“cut” the restriction sites on DNA strands based upon specific 3D structure

  11. Sticky Ends Allow Restriction Enyzmes To Assist In The Creation Of Recombinant DNA

  12. Boyer & Cohen (1973) – First to create recombinant DNA using restriction enzymes

  13. Splicing The Genes Into The Plasmid

  14. pGLO Plasmid

  15. APPLICATIONS OF RESTRICTION ENDONUCLEASES • Genetic Recombination/Creating of Genetically Modified Organsisms (GMO’s) • DNA Fingerprinting • Polymerase Chain Reactions (PCR) • Microarray Chips Contructed Using Genome Wide Association Studies (GWAS)

  16. PART ONE: DNA FINGERPRINTING

  17. PART 2 Polymerase Chain Reactions

  18. Why Use PCR? • Allows for amplification of genes for use in recombinant DNA (gene splicing) or isolation of sequences in DNA fingerprinting

  19. Required Ingredients For PCR • DNA • Primers (short sequences) • Taq Polymerase • Thermal Cycler

  20. PART 3: Microarrays and GWAS

  21. PART FOUR: CLONING

More Related