Islet-infiltrating B-Cells in Nonobese Diabetic Mice Predominantly Target Nervous System Elements Jorge Carrillo, Maria Carmen Puertas, Aurora Alba, Rosa Maria Ampudia, Xavier Pastor, Raquel Planas, Nadal Riutort, Nuria Alonso, Ricardo Pujol-Borrell, Pere Santamaria, Marta Vives-Pi, and Joan Verdaguer
Presented by Lei Lin and Aron Airall
obtained 352 hybridomas,
74 were Ab-secreting
(no diabetes or insulitis)
(Presence of mAbs to nuclear Ags and native DNA were confirmed by staining Hep-2 and Crithidia luciliae )
=> suggesting reactivity against pancreatic nervous system.
Abs from the hybridomas are specific for insulin, somatostatin, and glucagon
A - intraislet
B - neuroendocrine
C - mixed (islet)
H173- colocalize with Abs specific for GFAP, neurofilament, and peripherin
2 nervous system staining patterns:
H184- colocalize with Abs specific for GFAP, neurofilament, and peripherin
H122- colocalize only partially with Abs specific for GFAP
-52 Ab secreting hybridomas tested, 20 produced IgM mAb, 3 IgG1, 9 IgG2c, 19 IgG2b, and 1 IgA.
-IgM is the prevailing isotype for hybridomas restricted to exocrine, nuclear, and extracellular connective tissues => they originated from B cells producing polyreactive natural Abs.
-Most islet and nerve specific hybridomas secreted IgG2b, IgG2c, and IgG1 Abs => they derived from precursors that had undergone class switch recombination
=> Most islet-associated, Ab-secreting hybridomas specific for pancreatic islets and nervous system derive from B cells that have undergone Ig class switch recombination