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Immune parametres of mussels treated with Prestige fuel oil

Immune parametres of mussels treated with Prestige fuel oil. M. Camino Ordás, Joan Albaiges, Josep M. Bayona, Amando Ordás and Antonio Figueras. Instituto de Investigaciones Marinas (Vigo) Centro de Investigación y Desarrollo (Barcelona) Misión Biológica de Galicia (Pontevedra).

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Immune parametres of mussels treated with Prestige fuel oil

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  1. Immune parametres of mussels treated with Prestige fuel oil M. Camino Ordás, Joan Albaiges, Josep M. Bayona, Amando Ordás and Antonio Figueras Instituto de Investigaciones Marinas (Vigo) Centro de Investigación y Desarrollo (Barcelona) Misión Biológica de Galicia (Pontevedra)

  2. Immune system of bivalve molluscs Inespecific system  no antibody production • External barriers • Hemocytic infiltration Cellular defense Humoral defense HEMOCYTES SERUM SOLUBLE FACTORS • Phagocytosis • Respiratory burst • NO production • Lysozyme • Lectins • Prophenoloxidase

  3. contaminants depression in immune system infection by pathogens MORTALITY important economical losses in Galicia, one of the main Mediterranean mussel producers worldwide Objective of this work To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.

  4. contaminants depression in immune system infection by pathogens MORTALITY important economical losses in Galicia, one of the main Mediterranean mussel producers worldwide Objective of this work To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.

  5. contaminants depression in immune system infection by pathogens MORTALITY important economical losses in Galicia, one of the main Mediterranean mussel producers worldwide Objective of this work To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.

  6. contaminants depression in immune system infection by pathogens MORTALITY important economical losses inGalicia, one of the main Mediterranean mussel producers worldwide Objective of this work To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.

  7. contaminants depression in immune system infection by pathogens MORTALITY important economical losses in Galicia, one of the main Mediterranean mussel producers worldwide Objective of this work To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.

  8. Methodology tank 1 tank 2 tank 3 Monthly sampling during four months +1 kg fuel +2 kg fuel control • Histology. • Immune parametres: • Cellular: hemocyte viability, phagocytosis, NO production, chemiluminescence (CL). • Humoral: protein concentration and lysozime activity. • PAH concentration in water and mussel tissue (gills and digestive gland).

  9. Results 1. HISTOLOGY - No granulocytomes or neoplasia - Mytilicola in 68% of sampled animals

  10. df Viab. Phagoc. CL NO Protein Lisoz. Month (M) 3 0.008**252.75 0.0003** 5273.16**0.043 70.41** Treatment (T) 2 0.002 266.59 0.0001* 536.08 0.081* 54.98** M  T 6 0.008**143.37 0.0002** 2611.54*0.010 24.92* Error 36 0.002 146.12 0.0000 1015.16 0.020 8.65 2. IMMUNE PARAMETRES 2.1. Mean squares (34 factorial ANOVA): • No significant differences in phagocytic activity and significant differences in protein concentration. • Remaining parametres: interaction between month and treatment.

  11. Treatment Viab. Phagoc. CL NO ProteinLysoz. 2 kg 0.220a 11.080a 0.015a 52.770a 0.242a7.507ab 1 kg 0.219a 13.890a 0.013a 50.120a 0.283ab9.636a 0 kg 0.236a 19.108a 0.010a 61.200a 0.381b5.943b 2.2. One way ANOVA (without taking into account the sampling month): significant differences in protein concentration and lysozyme activity. 2.3. Least square means: Means followed by the same letter in a column are not statistically different according to the Waller-Duncan k- ratio t Test.

  12. Prestige fuel oil 3. PAH CONCENTRATION Similar methylphenantrene profiles in mussel organs and Prestige fuel oil  real exposure to the fuel.

  13. Higher TPAH concentration (ng/ g) in digestive gland than in gill. • Low TPAH conc. in control mussels. • Excepting gill from the first month, organs treated with 2 kg of fuel show higher TPAH conc. than 1 kg- treated ones. TPAH gill TPAH dig. gland

  14. REGRESSION PROCEDURE: •Model: [contam]=0 + 1  MONTH + 2  TREATMENT • Number of PAHs depending on each independent variable: ^ For comparisons between [PAH] and immune parametres, it would be more appropriate to use data from digestive gland, since [PAH] in gill is independent of our experimental design. Future analysis Comparison between gland [PAH] and immune data (difficult due to the experimental design).

  15. Muchas gracias

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