serial dilutions n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Serial Dilutions PowerPoint Presentation
Download Presentation
Serial Dilutions

Loading in 2 Seconds...

play fullscreen
1 / 27

Serial Dilutions - PowerPoint PPT Presentation


  • 114 Views
  • Uploaded on

Serial Dilutions. Moist the paper towels with set amount of solution. Get and label your petri dishes. Cut two pieces of paper towel and insert one into bottom of petri dish . Add 10 to 15 seeds to each bag using forceps then put 2 nd moistened towel over the seeds .

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Serial Dilutions' - wren


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide2

Moist the paper towels with set amount of solution

Get and label your petri dishes

Cut two pieces of paper towel and insert one into bottom of petri dish

Add 10 to 15 seeds to eachbag using forceps then put 2nd moistened towel over the seeds

slide3

To make the NaClSTOCKsolution

- Add # grams of sodium chloride for percent to beaker and add water to 100 mL

4%NaClStock Solution

4grams NaCl + 100 ml water

slide11

Using a disposable pipette, add solution from stock solution to a petri dish + paper towel– keep track of how much you add because you will need to add the same volume to moisten the other petri dishes

slide25
Be sure you have labeled each petri dish
    • Name, % Salt Solution, Seed
  • Moisten with a measured volume of water the second paper towel and place of the seeds – Remember to use the same volume for each 2nd towel/petri dish combo
  • Put in dark area for about a week.
slide26
Data
  • Determine the percent dead for each concentration.
  • Graph with the % dead on the y-axis and concentration on the x-axis.
  • Determine the LD50 or the concentration where 50% of the seeds did not germinate
slide27

100

75

Percentage of population killed by a given dose

50

25

LD

50

0

8

10

6

14

12

2

4

16

Dose (hypothetical units)