1 / 25

King Lab Yearly Safety Review

King Lab Yearly Safety Review. by Cammie Haase-Pettingell for March 2005 Disclaimer- This is Jonathan King’s lab in Biology Dept. yearly safety review and not to be substituted for an initial entry orientation. Any questions should be directed to Cammie 3-3545. Points be covered.

winslow
Download Presentation

King Lab Yearly Safety Review

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. King Lab Yearly Safety Review by Cammie Haase-Pettingell for March 2005 Disclaimer-This is Jonathan King’s lab in Biology Dept. yearly safety review and not to be substituted for an initial entry orientation. Any questions should be directed to Cammie 3-3545

  2. Points be covered • Emergency Action Plan • Eating • Chemical • Radioactive • Biologicals, Blood Borne Pathogens, Infectious Agents • High Voltage CHP GM

  3. Responsibility • PI • Ensure that the lab is conducting in a safe manor • This is by the PI or by a representative the safety rep. • Safety Rep. • To conduct incoming safety orientation • Go to safety meetings • Update the lab on new policy • Individual • To follow the above policy CHP GM

  4. Basics • No Eating in lab • Can eat in • Tea room • Computer room , • hall and seminar room • No smoking except outside • No mouth pipeting • Story… CHP GM

  5. Evacuation Plan CHP GM

  6. Fire Close the lab doors • Pull the fire red fire alarm • Located by Stairwells , Elevators • Leave by stairwells • Get to a phone- dial x100 report fire • Don’t hang up till they have hung up • Not responsible to fight a fire CHP GM

  7. Unknown fire such as strange smell • If in doubt call x 100 • Pull fire alarm Other scenario • Look around • Call John Fuccilo to ask he know anything • Ex . melted stuff in autoclave CHP GM

  8. Evacuation • The equipment halls can’t hear the alarm • As exit ensure people know about the alarm • If can - put experiments to rest- not required you safety is more important • So firefighters • ie do not stumble upon a active electrophoresis. • Just in case not back for a while may not be ruined • Evacuate by stairwell -all are fire stairwells • Meet by the concrete post by bld 66 • even if you think I saw you Check in so we know no one is stuck in the building • Then go off for what ever. • Some one needs to go to the command post say we are all out • by the door toward Main St. - CHP GM

  9. Chemicals • MSDS see handout • Chemical safety web training • http://mitehs.traincaster.com/ • Spill kits • 68-340D behind door • 68-322 under sink CHP GM

  10. Hoods • Simple Laminar Flow Hood • Ensure the back “slot” in back is clear • have items on lifts so it is not blocked • Simple test for flow • to tape Kimwipe • If malfunctioning- inform fixit Zone -A http://web.mit.edu/facilities/www/ • Rooms air flow and temperature is dependant on the hood. CHP GM

  11. Spills • Chemical • Radioactivity • Biological • General • Clear Area • Get on appropriate safety gear • Determine the Scope • Use the appropriated cleaner • Dispose correctly • Look at the Red flip chart by phone CHP GM

  12. Chemical Spill • Evacuate area- put sign up • Done appropriate safety - lab coat, eye protection, appropriate gloves • Determine the necessary sorbant, • For acid base or solvent. • Pour around the outside of the spill - mix in Hold breath • I use the dust pan and broom sweep up. • Wipe up residue • Dispose of as waste CHP GM

  13. Chemicals and Medical Emergency • Do not block body showers • Eye wash put injured eye down flush for 15 min. • Eyewash or distilled wash with tubing • Needs to be tempered water • Cut and injury- should go to Medical • can be documented and treated properly • Someone collapses • Ensure you are not putting you self in danger • If can rise -call EMT X100 • EMT will be concern that they are not in danger • try to figure what happened CHP GM

  14. Remember • Emergency number X100 • If working alone at night • inform some one on the Hall • Or call campus police 3-1212 • Safety is important to everyone. CHP GM

  15. Chemical waste- • Waste needs a red tag • Must be closed when not in use. • Most is in electrophoresis room • Silver- silver stain • MEOH- • Gel drying fix. , Transfer solution • Ethidium bromide - • Liquid and tips • Agarose in the dark room • Once full date and should be picked up immediately by law 2 days • If can call don’t date till wedded in link on King links page http://web.mit.edu/environment/environmental/ehs_services/ehs_areas/hazardous_waste/forms/chem_collection.html CHP GM

  16. Sharps • Two types • Biological or not chemical waste-gray bin • If it is sharp from a biological lab it can not be just thrown out. • Chemical waste- with red tags • At the Bench • Need to have covers and be covered when not in use. • Labeled CHP GM

  17. Radiation ext. 3-2180 • We use 14C and 35S to label rapidly proteins • Disposal designated by 1/2 life • 20-120day 1/2 life -35 S- 88 day 1/2 life • Over 120 day 1/2 life 14C = 5730 yr 1/2 life • Type- dry (paper gloves), hi level aqueous, sharps/epi. • Radioactivity stocks • is stored in the locked refrig and freezer 68-346D • Most experiment are preformed in the 68-346D. • Geiger counters in each side of the lab. • Pancake probes more sensitive • Ensure the battery is off when done. CHP GM

  18. Radioactive spill • Clear the area- block off • Ensure they you have lab coat, safety glasses, double gloves, boots? • Monitor the area with Geiger counter • Mark the area- where there are counts • One does not inactivate the radioactive just clean off the solution. • Good agents -Bleach, Radiact wash, Am4OH • Clean till no signal • RPO will do wipe test • If still signal cover till decayed. CHP GM

  19. Biological • BL1- E. Coli • low BL2 - Salmonella typhurminium LT2 • Clean up • All bacteria needs to be in activated by before disposal or going to the kitchen • Autoclaving • Chemical, 10% bleach, 1% Westcodyne, Lysol • Waste • Plates go into the bucket autoclaved in big autoclave after kitchen hours. CHP GM

  20. Biological spills • Block the area • Let aerosol settle • Safety gear • Clean up by pour/spray disinfectant on glassware • with dusty pan • Put paper towel on it and pour/spray disinfectant on it • Dispose of in the waste. • Look for the new biological spill kits CHP GM

  21. Decontaminating the Bench • 70% ethanol is a better agent to decontaminate the bench than 100% ethanol • 70% is better at denaturing the outer membrane and subsequently lysing the cell • 100 % will only causes effects the on the outer membrane but not lysis. CHP GM

  22. High Voltage • Any voltage should be treated as if it is dangerous. • AC current is the most dangerous. • Running a PAGE • Start up a gel by connecting the black lead first as a ground and then the red lead. • It should be noted that high power leads should be used when running a sequencing gel with high voltage. • Turn off a power supply by turning down the voltage source, then turn off the power. • After turning off power, disconnect the red (positive) lead first. • If the hazard light is flashing on the power supply, turn it off and determine what the problem is and fix it, or have it fixed. • If someone is unconscious, call x100 which is the emergency phone number at MIT. CHP GM

  23. Chemical Hygiene Plan • OSHA right to know • MSDS know how to read • SOP- Standard Operating Procedures • a written procedure for use of every chemical CHP GM

  24. Safety is everyone responsibility • Everyone will be responsible for the level 1 check list which I email the pdf • keep check off for a month • Cammie and Shannon are the safety Reps to do an over view • ensure all unlabeled liquid containing item have a label can be cryptic CHP GM

More Related