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  1. PowerPoint File available: http://bl831.als.lbl.gov/ ~jamesh/powerpoint/ Oslo_2010.ppt

  2. Download Elves from: http://ucxray.berkeley.edu/~jamesh/elves

  3. Advanced Light Source

  4. George Meigs Acknowledgments ALS 8.3.1 Team Beamline 8.3.1 staff Jane Tanamachi

  5. Acknowledgements Ken Frankel Chris Neilson Michael Blum Joe Ferrara 8.3.1 PRT: Jamie Cate Center for Structure of Membrane Proteins Membrane Protein Expression Center II Center for HIV Accessory and Regulatory Complexes W. M. Keck Foundation Plexxikon, Inc. M D Anderson CRC University of California Berkeley University of California San Francisco National Science Foundation University of California Campus-Laboratory Collaboration Grant Henry Wheeler The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, Materials Sciences Division, of the US Department of Energy under contract No. DE-AC02-05CH11231 at Lawrence Berkeley National Laboratory.

  6. Elven Automation Elves examine images and set-up data processing Elves run… mosflm scala solve mlphare dm arp/warp

  7. Elven Automation Elves examine images and set-up data processing Elves run… mosflm scala solve mlphare dm arp/warp

  8. Elven Automation Elves examine images and set-up data processing Elves run… mosflm scala solve mlphare dm arp/warp

  9. Conversational User Interface user input->

  10. Conversational User Interface user input->process the data in /data/semet

  11. Conversational User Interface user input->process the data in /data/semet recognitionblah blah blah blah /data/semet

  12. Conversational User Interface user input->process the data in /data/semet recognitionblah blah blah blah /data/semet simplify /data/semet

  13. Conversational User Interface user input->process the data in /data/semet recognitionblah blah blah blah /data/semet simplify /data/semet preempt/data/semet contains image files

  14. Conversational User Interface user input->process the data in /data/semet report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 100 mm from the crystal using 1.54 Å x-rays.

  15. Conversational User Interface user input->process the data in /data/semet report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 100 mm from the crystal using 1.54 Å x-rays. confirm Everything look okay? [Yes] ->

  16. Conversational User Interface user input->process the data in /data/semet report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 100 mm from the crystal using 1.54 Å x-rays. confirm Everything look okay? [Yes] -> user inputdistance was more like 110

  17. Conversational User Interface user input->distance was more like 110

  18. Conversational User Interface user input->distance was more like 110 recognitiondistance blah blah blah 110

  19. Conversational User Interface user input->distance was more like 110 recognitiondistance blah blah blah 110 simplify DISTANCE 110

  20. Conversational User Interface user input->distance was more like 110 report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 110 mm from the crystal using 1.54 Å x-rays.

  21. Conversational User Interface user input->distance was more like 110 report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 110 mm from the crystal using 1.54 Å x-rays. confirm Everything look okay? [Yes] ->

  22. Conversational User Interface user input->distance was more like 110 report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 110 mm from the crystal using 1.54 Å x-rays. confirm Everything look okay? [Yes] user input -> wavelength is wrong

  23. Conversational User Interface user input->wavelength is wrong

  24. Conversational User Interface user input->wavelength is wrong recognitionwavelength = -NOT

  25. Conversational User Interface user input->wavelength is wrong recognitionwavelength = -NOT simplify WAVELENGTH

  26. Conversational User Interface user input->wavelength is wrong recognitionwavelength = -NOT simplify WAVELENGTH query What is the x-ray wavelength? [1.54Å] ->

  27. Conversational User Interface user input->wavelength is wrong recognitionwavelength = -NOT simplify WAVELENGTH query What is the x-ray wavelength? [1.54Å] user input -> 1

  28. Conversational User Interface user input->wavelength is wrong query What is the x-ray wavelength? [1.54Å] user input -> 1

  29. Conversational User Interface user input->wavelength is wrong query What is the x-ray wavelength? [1.54Å] user input -> 1 report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 110 mm from the crystal using 1 Å x-rays.

  30. Conversational User Interface user input->wavelength is wrong query What is the x-ray wavelength? [1.54Å] user input -> 1 report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 110 mm from the crystal using 1 Å x-rays. confirm Everything look okay? [Yes] ->

  31. Conversational User Interface user input->wavelength is wrong query What is the x-ray wavelength? [1.54Å] user input -> 1 report Elves will process from /data/semet/frame001.img to /data/semet/frame100.img Data were collected from 0º to 100º in 1º steps with an ADSC Q4 detector 110 mm from the crystal using 1 Å x-rays. confirm Everything look okay? [Yes] user input -> Yes

  32. Major Phasing techniques • Molecular Replacement • Multiple Isomorphous Replacement • Multiwavelength Anomalous Diffraction • Single-wavelength Anomalous Diffraction

  33. Molecular Replacement correct structure and intensities ? http://www.ysbl.york.ac.uk/~cowtan/fourier/coeff.html