Edvotek kit # 282. Principles of Enzyme Catalysis. Why?. For Biology II or AP biology. Follow up to:. Introduction to. Protein structure & function Properties of enzymes Factors that effect proteins. How do enzymes work/ How is enzyme activity measured?
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Follow up to:
We will measure the amount of H2O2 remaining after catalysis by catalase by coupling to a secondary reaction with KI as follows:
2 I- + H+ + H2O22H2O + I2
(colorless) (reddish brown)
Then you will take aliquots out of the reaction tube at 6 different time intervals and mix with an assay solution.
The assay solution does two things
Stops the action of catalase, so no more H2O2 is broken down
Contains the acidic KI that can react with the remaining H2O2How So?You will catalyze H2O2 with catalase in a reaction tube.
Materials for each group
- 6mL reaction cocktail (buffered H2O2)
Prepare tubes as directed
p. 10-12 labeled as follows
For spectrophotometer readings