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ENZYME CATALYSIS

BIOCHEMISTRY

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ENZYME CATALYSIS

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  1. M.Prasad Naidu MSc Medical Biochemistry, Ph.D.Research Scholar Enzyme Kinetics and Catalysis

  2. Kinetics of Enzymes Enzymes follow zero order kinetics when substrate concentrations are high. Zero order means there is no increase in the rate of the reaction when more substrate is added. Given the following breakdown of sucrose to glucose and fructose Sucrose + H20 Glucose + Fructose

  3. E = Enzyme S = Substrate P = Product ES = Enzyme-Substrate complex k1 rate constant for the forward reaction k-1 = rate constant for the breakdown of the ES to substrate k2 = rate constant for the formation of the products

  4. When the substrate concentration becomes large enough to force the equilibrium to form completely all ES the second step in the reaction becomes rate limiting because no more ES can be made and the enzyme-substrate complex is at its maximum value. [ES] is the difference between the rates of ES formation minus the rates of its disappearance. 1

  5. Assumption of equilibrium k-1>>k2 the formation of product is so much slower than the formation of the ES complex. That we can assume: Ks is the dissociation constant for the ES complex.

  6. Assumption of steady state Transient phase where in the course of a reaction the concentration of ES does not change 2

  7. 3 Combining 1 + 2 + 3 rearranging Divide by k1 and solve for [ES] Where

  8. vo is the initial velocity when the reaction is just starting out. And is the maximum velocity The Michaelis - Menten equation

  9. The Km is the substrate concentration where vo equals one-half Vmax

  10. The KM widely varies among different enzymes The KM can be expressed as: As Ks decreases, the affinity for the substrate increases. The KM can be a measure for substrate affinity if k2<k-1

  11. There are a wide range of KM, Vmax , and efficiency seen in enzymes But how do we analyze kinetic data?

  12. The double reciprocal plot

  13. Lineweaver-Burk plot: slope = KM/Vmax, 1/vo intercept is equal to 1/Vmax the extrapolated x intercept is equal to -1/KM For small errors in at low [S] leads to large errors in 1/vo kcat is how many reactions an enzyme can catalyze per second The turnover number

  14. For Michaelis -Menton kinetics k2= kcat When [S] << KM very little ES is formed and [E] = [E]T and Kcat/KM is a measure of catalytic efficiency

  15. What is catalytic perfection? When k2>>k-1 or the ratio is maximum Or when every substrate that hits the enzyme causes a reaction to take place. This is catalytic perfection. Then Diffusion-controlled limit- diffusion rate of a substrate is in the range of 108 to 109 M-1s-1. An enzyme lowers the transition state so there is no activation energy and the catalyzed rate is as fast as molecules collide.

  16. Reaction MechanismsA: Sequential Reactions • All substrates must combine with enzyme before reaction can occur

  17. Bisubstrate reactions

  18. Random Bisubstrate Reactions

  19. Ping-Pong Reactions • Group transfer reactions • One or more products released before all substrates added

  20. Kinetic data cannot unambiguously establish a reaction mechanism. Although a phenomenological description can be obtained the nature of the reaction intermediates remain indeterminate and other independent measurements are needed.

  21. Inhibition kinetics • There are three types of inhibition kinetics competitive, • mixed and uncompetitive. • Competitive- Where the inhibitor competes with the substrate.

  22. Competitive Inhibition

  23. HIV protease inhibitors

  24. Competitive Inhibition: Lineweaver-Burke Plot

  25. Uncompetitive Inhibition

  26. Uncompetitive Inhibition: Lineweaver-Burke Plot

  27. Mixed inhibition

  28. Mixed inhibition is when the inhibitor binds to the enzyme at a location distinct from the substrate binding site. The binding of the inhibitor will either alter the KM or Vmax or both.

  29. The effect of pH on kinetic parameters

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