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Microhematocrit Determination. Microhematocrit. Hematocrit Test that provides a health care worker with an estimate of the patient’s red cell volume. Remember that the RBCs determine a person’s oxygen capacity. A hematocrit measurement is used to screen for anemia, blood

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Microhematocrit Determination


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microhematocrit
Microhematocrit
  • Hematocrit
  • Test that provides a health care worker with an estimate of the

patient’s red cell volume.

  • Remember that the RBCs determine a person’s oxygen capacity.
  • A hematocrit measurement is used to screen for anemia, blood

donation, evaluating therapies and treatment, as well as assessing

blood loss.

  • A hematocrit can be measured manually (microhematocrit) or by using a hematology analyzer.
  • A hematocrit is included as part of a CBC (complete blood count).
microhematocrit1
Microhematocrit
  • Microhematocrit
  • Test involves only a small amount of blood.
  • The test uses the separation of cellular components by

centrifugation.

  • Whole blood is centrifuged in a capillary tube.
  • Red cells settle at the bottom with the buffy coat

(WBCs and PLTs) resting just above the RBCs.

  • The remaining liquid is plasma.
  • The microhematocrit is determined by comparing the

volume of RBCs to the total volume of the whole blood

sample.

  • This number is expressed in a percentage.
  • A microhematocrit is also known as a crit or Hct.
microhematocrit equipment reference values
Microhematocrit Equipment/Reference Values
  • Microhematocrit Equipment
  • Microhematocrit centrifuge
  • Microhematocrit tubes
  • Capillary stick supplies
  • Hematocrit Reference Values
  • Like hemoglobin, hematocrit values differ based on age

and gender.

  • Adult males range from 42-52 %
  • Adult females range from 36-48 %
  • Newborns range from 51-61 %
microhematocrit reference values
Microhematocrit Reference Values
  • Hematocrit Values
  • Hematocrits below normal can point to……
  • Anemia
  • Current bleeding
  • Hematocrits above normal can point to…..
  • Dehydration
  • Polycythemia
  • In a healthy person the hematocrit should be equal to the hemoglobin multiplied by 3.
  • Example: If you have a hemoglobin of 15 g/dL your

hematocrit should be around 45%.

performing a microhematocrit
Performing a Microhematocrit
  • Performing a Microhematocrit
  • The sample of blood can be collected from a capillary

puncture or from an EDTA tube collected from a

venipuncture.

  • Capillary blood should be collected into a heparinized

capillary tube.

  • All blood needs to be mixed thoroughly.
  • Clay can be used to seal the capillary tube or self

sealing tubes can be used instead.

  • Sealed tubes need to be centrifuged in a

microhematocrit centrifuge.

reading a microhematocrit
Reading a Microhematocrit
  • Reading a Microhematocrit
  • You can determine the percent of RBCs in the sample

by using a microhematocrit reader.