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Heparin Blocking of Virus Entry into PAEC-KO Cells

TB40/E preincubated with increasing heparin concentrations to block virus entry into porcine aortic endothelial cells-KO. Results show mean percent of infected cells. 8

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Heparin Blocking of Virus Entry into PAEC-KO Cells

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  1. Supplemental Figure 1, Ghielmetti et al SUPPLEMENTAL FIGURE 1. To determine the concentration required for complete blocking of virus entry, TB40/E was preincubated with increasing concentration of heparin ranging from 2 mg/ml to 20 mg/ml, before being added to PAEC-KO. To verify that heparin was binding only on the virus side, porcine aortic endothelial cells-KO were preincubated with 20 mg/ml heparin before the addition of the virus. The values represent the mean percent of infected cells, as counted from 6 different fields in one representative experiment.

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