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Separation of Urinary Pyridinium Crosslinks by High-Performance Liquid Chromatography

Explore the separation of pyridinoline and deoxypypridinoline with an internal standard using fluorescence detection at 295/400 nm. Time points at 0, 2, 4, 6, 8, 10, and 12 minutes are analyzed. Data by Kraenzlin et al. Supplementary Figure 1.

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Separation of Urinary Pyridinium Crosslinks by High-Performance Liquid Chromatography

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  1. µV 2.0e+5 PYD 1.5e+5 ISTD Fluorescence (295/400 nm) 1.0e+5 DPD 0.5e+4 0.0 -0.5e+4 2 4 6 8 10 12 Time (min) Kraenzlin et al. Supplemental Figure 1 Supplemental Figure 1: Separation of urinary pyridinium crosslinks, pyridinoline (PYD) and deoxypypridinoline (DPD), by reverse phase high performance liquid chromatography;(ISTD, internal standard)

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