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Genome-wide Survey of Cellulase Genes in White Rot Fungus

This poster presentation at Kochi University of Technology explores the cellulase-related genes in Pleurotus ostreatus. The study aimed to identify endocellulase genes in the fungus by utilizing amino acid sequence data, CODEHOP primers, PCR amplification, and sequence analysis.

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Genome-wide Survey of Cellulase Genes in White Rot Fungus

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  1. RG/WP Poster presenters Wood protection

  2. Poster 398 Tomoyuki TAMENORI, Sakae HORISAWA Genome-wide survey of cellulase related gene of white rot fungus, Pleurotus ostreatus Kochi University of Technology Tomoyuki Tamenori 185 Miyanokuchi, Tosayamada, City Kami-city, Kochi JAPAN

  3. Poster 398 Genome-wide survey of cellulase related gene of white rot fungus, Pleurotus ostreatus Aim of work • Genes of Pleurotus ostreatus encoding endocellulases were searched based on the information regarding the cellulases of other organisms.

  4. Poster 398 Results • Amino acid sequence data of cellulase (mainly endoglucosidase) were obtained from the biological databases. • Consensus-degenerate hybrid oligonucleotide primers (CODEHOP) were designed . • PCR amplification and sequence analysis were conducted.

  5. Poster 398 Results • PCR amplification was then performed using each pair of CODEHOPs. Sequencing

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