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Expression of EpCOL Genes in Various Tissues Using RT-PCR

This study detected EpCOL1 to EpCOL12 expression in different tissues using Total RNA isolated from floral buds, peduncles, capsules, leaves, and roots as templates for semi-quantitative RT-PCR. The experiment was conducted four times with consistent outcomes. RNA quality control was confirmed by amplifying E. pusilla Actin.

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Expression of EpCOL Genes in Various Tissues Using RT-PCR

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  1. Supplementary file 8. Detection of expression of EpCOL1 to EpCOL12 in different tissue Total RNA isolated from several different tissues, including floral bud/opened flower (F), peduncles (P), capsules (C), leaves (L) and roots (R) were used as templates for semi-quantitative RT-PCR. This experiment was repeated four times with similar results. Loading and RNA quality control were verified by amplification of the constitutive E. pusilla Actin. F P C L R

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