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Engr 1182 Nano Lab

This presentation outlines the overall procedure for demolding PDMS lids, aligning chip holders, and testing fluidics within chips. Begin by demolding two PDMS lids, ensuring one stays in its petri dish for further use. Follow the specified cutting and hole-punching instructions, cleaning all components with an ultrasonic cleaner. Proper alignment during chip assembly is crucial, so take adequate time for precision. Finally, perform fluidics testing by pumping fluid through staging wells, documenting observations and notes for future reference.

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Engr 1182 Nano Lab

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  1. Engr1182 Nano Lab Demolding Presentation Short Title

  2. Overall Procedure for Today • Demold two of the PDMS lids. Keep one in its petri dish for second chip. • Cut and punch holes as instructed in the procedure (make sure to use 2mm punch size) • Clean chipholder and PDMS Lid and acrylic chips with the ultrasonic cleaner • Dry and assemble, avoiding contaminating contact with other surfaces • NOTE: Wear Gloves to avoid contaminating the chip and each team will divide in half and demold the two lids. Presentation Short Title

  3. Chip preparation • It is very important that you align the chipholder and chip carefully. Take as much time as you need to get this right! • Print a 1:1 drawing of your chip design. • Align chip holder over printout • Align and place detection and staging wells of acrylic chip over printout of wells • Align and place lid access holes over wells • Assemble chipholder top Presentation Short Title

  4. Fluidics testing • Insert the 3 ml luer lock syringes with cut tapered tip into access holes and waste well. • Pump fluid into and through staging wells. Suction can be applied at the waste well. • Pump dyed water into a staging well and then further into the detection well. Determine best channels/wells to use for final test. • Write up NOTES on your observations and comments on your design. Store in your notebook. Presentation Short Title

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