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Engr 1182 Nano Lab

Engr 1182 Nano Lab. Detection Circuit 2. Circuit Schematic. Detection Alignment Device (DAD). Chip Holder Alignment Holes. Detector Slot. LED Chamber. DAD Connection.

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Engr 1182 Nano Lab

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  1. Engr 1182 Nano Lab Detection Circuit 2 Presentation Short Title

  2. Circuit Schematic Presentation Short Title

  3. Detection Alignment Device (DAD) Chip Holder Alignment Holes Detector Slot LED Chamber Presentation Short Title

  4. DAD Connection • After completing the circuit testing from Lab 3, connect the detection alignment device (DAD) in place of the potentiometer. Be sure to measure the 47 Ohm resistor. Be careful with polarity (instructors need to check before applying power!) • Connect extender leads from DAD LED and photodetector to appropriate pins of prototype board. Silver mark on leads is ground connection. • Do not touch the electronics, including the leads from the DAD, without attaching the wrist straps!!! Presentation Short Title

  5. DAD VERIFICATION Photo- Transistor 3 wires Fluorescein Detection Circuit Diode 2 wires Presentation Short Title Check DAD- Polarity is critical. Have instructors check. P. 5 DAD

  6. Integration and Calibration • Clean and place chip with chipholderon DAD. Choose placement for best alignment of photodetector under detection well. Rotate for best alignment. Don’t forget to put a drop of water between the chip and the chipholder bottom. • Obtain DI water and 5ml sample of 1000 ppm fluorescein from front of lab. • Create 4 samples of fluorescein using your syringes and the glass vials provided. The three samples should be 750 ppm, 500 ppm, 250 and 750 ppm. These will be re-used in future labs. Mark their concentrations. Presentation Short Title

  7. Calibration • First use DI water to fill the detection well in the chip. Cover with bucket and obtain the LED readout. This is your baseline value (ideally 3-7). • For each sample fill the detection well, cover DAD, obtain corresponding LED readout. Repeat this step for each sample as time allows and record on your lab worksheet. Average the values for each concentration. Construct a calibration curve of average LED readout vs. concentration. Presentation Short Title

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