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DERIVATION OF ILS & ISS

LXS RI PANEL: LARGEST EXTANT RI SERIES 78 STRAINS BETH BENNETT T.E. JOHNSON UNIV OF COLORADO BOULDER, CO. DERIVATION OF ILS & ISS. 8 WAY CROSS: A, AKR, BALB, C3H, C57, DBA, ISBI, RIII. HETEROGENOUS STOCK (HS). Select for differential ethanol sensitivity for 25 generations. LS & SS.

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DERIVATION OF ILS & ISS

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  1. LXS RI PANEL:LARGEST EXTANT RI SERIES78 STRAINS BETH BENNETTT.E. JOHNSONUNIV OF COLORADOBOULDER, CO

  2. DERIVATION OF ILS & ISS 8 WAY CROSS: A, AKR, BALB, C3H, C57, DBA, ISBI, RIII HETEROGENOUS STOCK (HS) Select for differential ethanol sensitivity for 25 generations LS & SS Inbreed 20 generations ILS & ISS

  3. RI CONSTRUCTION

  4. BODY WT IN FEMALES • Female weight chosen as mapping character • Not selected in progenitor strains • Possible interaction with fertility, lifespan • Easily measured test phenotype for LXS RI • Measured at 30 day intervals in generation 13 • 94% inbred, 89% loci fixed • Heritability estimated • QTLs mapped using MapManagerQT

  5. GenotypingR.Williams and L. Lu • Begun at N18 • All Parents within Strain Genotyped • Expect 96% of Loci Fixed • 361 Polymorphic Markers Identified • Approximate Marker Density of 4.5 cM

  6. 30 DAY WEIGHTS

  7. WT DISTRIBUTION AT 60, 90, 120, 150 DAYS

  8. 30 Day 60 Day 90 Day 120 Day 150 Day 30 Day .49 .64 .53 .57 .56 60 Day .57 .62 .79 .83 .80 90 Day .49 .88 .66 .86 .91 120 Day .53 .83 .90 .64 .95 150 Day .53 .84 .89 .92 .72 Heritability (h2),Genetic correlation (rG), and Phenotypic correlation (rP)

  9. FEMALE BODY WEIGHT IN LXS RIs AT 30 DAY INTERVALS WT IN GRAMS

  10. A. 30-60 Days B. 60-90 Days 120 200 180 100 160 140 80 120 100 60 80 40 60 40 20 20 0 0 -1.25 -.75 -.25 .25 .75 1.25 1.75 2.25 2.75 3.25 3.75 -1.25 -.75 -.25 .25 .75 1.25 1.75 2.25 2.75 3.25 3.75 -1.00 -.50 0.00 .50 1.00 1.50 2.00 2.50 3.00 3.50 -1.00 -.50 0.00 .50 1.00 1.50 2.00 2.50 3.00 3.50 D. 120-150 Days C. 90-120 Days 100 120 100 80 80 60 N of Mice N of Mice 60 40 40 20 20 0 0 -1.25 -.75 -.25 .25 .75 1.25 1.75 2.25 2.75 3.25 3.75 -1.25 -.75 -.25 .25 .75 1.25 1.75 2.25 2.75 3.25 3.75 -1.00 -.50 0.00 .50 1.00 1.50 2.00 2.50 3.00 3.50 -1.00 -.50 0.00 .50 1.00 1.50 2.00 2.50 3.00 3.50 Gms Gained per Week Gms Gained per Week GROWTH RATE AT 30 DAY INTERVALS

  11. Interval (Days) Mean Growth Rate + SE N strains h2 (95% CI) 30-60 1.20 + 0.004 97 .48 (.39-.56) 60-90 0.49 + 0.003 86 .42 (.32-.50) 90-120 0.37 + 0.003 75 .25 (.15-.35) 120-150 0.25 + 0.002 63 .22 (.09-.33) h2 for Growth Rate

  12. QTL MAPPING FOR BODY WT AT 30 DAYS

  13. QTL MAPPING FOR BODY WT AT 60 DAYS LOD=3.4

  14. QTL MAPPING FOR BODY WT AT 90, 120 & 150 DAYS LOD=3.2

  15. PHENOTYPES BEING ASSESSED IN LXS RI • ETHANOL STUDY: • Ethanol sensitivity & blood ethanol at awakening • Ethanol tolerance • Low dose ethanol activation • AGING STUDY: • Lifespan • Dietary restriction responses • Torpor, Food processing efficiency, Hair growth rate

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