1 / 4

Ochratoxin A (OTA)

Determination of ochratoxin A in wine by means of immunoaffinity column clean-up and high-performance liquid chromatography. Toxicity OTA is hepatotoxic, nephrotoxic, teratogenic and carcinogenic to animals

toviel
Download Presentation

Ochratoxin A (OTA)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Determination of ochratoxin A in wine by means of immunoaffinity column clean-up and high-performance liquid chromatography • Toxicity • OTA is hepatotoxic, nephrotoxic, teratogenic and carcinogenic to animals • OTA was classified by the International Agency for Research on Cancer (IARC) as a possible human carcinogen (group 2B) Regulatory limits set by the European Union • COMMISSION REGULATION (EC) No 123/2005 • 2,0 ppb for wine (red, rose and white) Ochratoxin A (OTA)

  2. Sample preparation and clean-up Method • Chromatographic determination • The clean-up procedure for ochratoxin A involves generally IAC, SPE • Immunoaffinity chromatography (IAC) clean-up is based on the binding interaction of ochratoxin A-specific antibodies and has been set as European Standard (SR EN 14133) for ochratoxin A analysis in wine and beer Immunoaffinity column design containing solid extraction phase made from antibody-modified support beads binding ochratoxin A

  3. Sample preparation and immunoaffinity clean-up

  4. HPLC determination of ochratoxin A • Chromatographic conditions: • OTA was quantified by reverse phase high–performance liquid chromatography (HPLC) with fluorescence detection (excitation wavelength 330nm, emission wavelength 450nm). • mobile phase consisted of a mixture of acetonitrile–water–acetic acid (99:99:2) • analytical column were reverse phase, C-18 with 5 μm particles • flow-rate 1 mL/min • Injection 20 yL Chromatogram of OTA contaminated red wine

More Related