Chapter 15

1. Chapter 15 Commercial Microbial Products and Biotechnology

3. Primary and Secondary Metabolites Yeast Fermentation Antibiotic Production: P. chrysogenum Clicker Question:

4. Primary and Secondary Metabolites Yeast Fermentation Antibiotic Production: Penicilliumchrysogenum Clicker Question:

5. Primary and Secondary Metabolites Yeast Fermentation Antibiotic Production: Penicilliumchrysogenum Clicker Question:

6. Fermenters

7. Research to Industrial Size Fermenters

9. Finding Antibiotic Producers

10. After Finding an Antibiotic Producing Microbe Test for antibiotic yield…need high yielding strains to produce enough for the next steps. Purification so that a highly pure crystalline product. Chemical Identification, and testing tolerance in animal models.

11. After Finding an Antibiotic Producing Microbe Test for antibiotic yield…need high yielding strains to produce enough for the next steps. Purification so that a highly pure crystalline product. Chemical Identification, and testing tolerance in animal models. Clicker Question:

12. Industrial Production of Penicillin

13. Fermenter Kinetics with Penicilliumchrysogenum Clicker Question:

14. Fermenter Kinetics with Penicilliumchrysogenum Clicker Question:

15. Fermenter Kinetics with Penicilliumchrysogenum Clicker Question:

16. Fermenter Kinetics with Penicilliumchrysogenum Clicker Question:

17. Tetracycline Production

18. Vitamin Production

19. A

21. Extremozymes (a) Acid Tolerant Enzymes to Digest (b) Pullulanase from Fibrous Foods Pyrococcuswoesei which has a growth opt temp = 100oC

22. Immobilized Enzymes: Reactor Columns

23. Wine Fermentation 1. It starts with the type of grape, Vitisvinefera. 2. The yeast: Saccharomyces ellipsoideus. 3. Then finished with the malolactic fermentation (acid tolerant Oenococcus, Lactobacillusand Pediococcus).

24. Vitisvinefera, what a beautiful plant! Sauvingon Blanc Cabernet Sauvingon Pinot Noir Cultivars

25. Grape Chemistry Wines have a pH ~3.5 Stored as sugar or amino acid conjugates – liberated during crushing and enzymatic attack during fermantation Science, 10 February 2006 vol 311 pg 804

26. Aging Barrels in Beringer Winery Caves Malolactic Fermentation: Convert Malate  Lactate + CO2

27. Uncontrolled vs Controlled Fermentation The Evolution of Wine Making ? How did it start? - required non-leaking vessels, and - making juice…with the discovery that old juice became a bit different, and what seemed to be a great idea. Clicker Question:

28. Uncontrolled vs Controlled Fermentation The Evolution of Wine Making ? How did it start? - required non-leaking vessels, and - making juice…with the discovery that old juice became a bit different, and what seemed to be a great idea. Some how – they could control the fermentation by using the gooey stuff at the bottom of the barrel to start the next round of fermentation. This reduced tremendous variation in batches. This continued for a few thousands of years. Then came the pure culture technique (~1880’s)

29. Wine is Good for You

30. Beer is Made from Malted Barley “Beer is proof the God loves us and wants us to be happy” ….Benjamin Franklin

31. Mashing – Temp RegulationMaking the WORT

32. Hops

33. Beer

34. Home Brew Boiling the Wort Fermentation Bottling Pils Dark

35. Whiskey’s Barley or Grape Fermentation  Distilled, usually to 100 proof (50% ethanol). Distillate  Aging Barrels (oak) for 10-12 years ending up about 80 proof along with a great change in flavor. Barley fermentation (beer) distillate  aging to make Scotch. Barley, Corn + Rye (beer) distillate  aging in charred barrels = Bourbonif done in the State of Kentucky Others named Whiskey if done out of Kentucky. Barley, Wheat + Rye (beer) distillate  aging to make Irish Whiskey Barley + Rye (beer) distillate  aging to make Canadian Whiskey.

36. Brandies and Vodkas Grape wines distillate  aging to Cognac (only in the Cognac Department of France), Brandies everywhere else. Classical Vodka from potato….with little flavor other than ethanol. New trendy vodkas:

37. Ethanol as a Biofuel Corn based Ethanol Switch Grass Clicker Question:

38. Ethanol as a Biofuel Corn based Ethanol Switch Grass Clicker Question:

39. Ethanol as a Biofuel Corn based Ethanol Switch Grass Clicker Question:

40. Ethanol as a Biofuel Corn based Ethanol Switch Grass Clicker Question:

41. Oil from Algae Botryococcusbraunii. The oil droplets are evident in this phase micrograph, some have been expelled and are droplets in the medium. These oil droplets have 30-36 carbon hydrocarbons. Clicker Question:

42. Expressing Mammalian Genes in Bacteria There are a few Problems … that can be overcome: Expressing intron-free genes in bacteria. Clicker Question:

43. Expressing Mammalian Genes in Bacteria There are a few Problems … that can be overcome: Expressing intron-free genes in bacteria. Clicker Question:

44. Expressing intron-free genes in bacteria. Clicker Question:

45. Expressing intron-free genes in bacteria. Clicker Question:

46. Expressing Mammalian Genes in Bacteria • There are a few Problems … that can be overcome: • Expressing intron-free genes in bacteria. • Purification of the Protein. • Is it expressed in the cytoplasm or exported to the medium • Fusion proteins and other ways to simplfy purification. • Testing the purity AND safety.

48. Recombinant Vaccines Pathogenic Bacteria and Viruses have many genes, a great diversity of proteins. Clicker Question:

49. Recombinant Vaccines Pathogenic Bacteria and Viruses have many genes, a great diversity of proteins. Clicker Question:

50. Transgenic Animals and Plants Transgenic salmon is 4.5 kg. Control salmon is 1.5 kg.