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Genetic engineering. Genetic engineering through selective breeding Ex. Dogs, Agricultural products- what else? Selective breeding leads to inbreeding: mating between 2 closely related individuals. Selective breeding takes many generations

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Presentation Transcript
slide2
Genetic engineering through selective breeding
  • Ex. Dogs, Agricultural products- what else?
  • Selective breeding leads to inbreeding: mating between 2 closely related individuals
slide3
Selective breeding takes many generations
  • Genetic engineering is a faster way of increasing a specific gene in a population
  • A.K.A recombinant DNA technology
slide4
Recombinant DNA-recombining fragments of DNA from different sources
  • DNA from one organism inserted into the DNA of a second organism- known as transgenic
transgenic organisms contain genes from other species
Transgenic organisms- contain genes from other species
  • Tobacco plant with firefly glow gene
  • Bacteria plasmids with human genes
slide6
1. Isolate DNA to be inserted
  • 2. Attach DNA to an insertion “vehicle”
  • 3. Transfer vehicle to host organism
restriction enzymes
Restriction Enzymes
  • Cut DNA AT SPECIFIC SITES
  • Different restriction enzymes cut at different places.
  • Example EcoR1
slide8
Use restriction enzyme to cut DNA
  • “Glue” sticky ends together with ligase
slide10
Extra circular DNA in bacteria
  • Move from bacteria to bacteria
    • Transformation
  • Transfer genetic information
slide12
Transgenic bacteria
    • Contain human genes
    • Express human proteins
  • Easy and cheap to grow
  • Many possibilities
homework
Homework
  • Read pgs 349-356. Answer Section Assessment questions 1-5