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Genetic engineering

Genetic engineering

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Genetic engineering

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  1. Genetic engineering

  2. Genetic engineering through selective breeding • Ex. Dogs, Agricultural products- what else? • Selective breeding leads to inbreeding: mating between 2 closely related individuals

  3. Selective breeding takes many generations • Genetic engineering is a faster way of increasing a specific gene in a population • A.K.A recombinant DNA technology

  4. Recombinant DNA-recombining fragments of DNA from different sources • DNA from one organism inserted into the DNA of a second organism- known as transgenic

  5. Transgenic organisms- contain genes from other species • Tobacco plant with firefly glow gene • Bacteria plasmids with human genes

  6. 1. Isolate DNA to be inserted • 2. Attach DNA to an insertion “vehicle” • 3. Transfer vehicle to host organism

  7. Restriction Enzymes • Cut DNA AT SPECIFIC SITES • Different restriction enzymes cut at different places. • Example EcoR1

  8. Use restriction enzyme to cut DNA • “Glue” sticky ends together with ligase

  9. Extra circular DNA in bacteria • Move from bacteria to bacteria • Transformation • Transfer genetic information

  10. Transgenic bacteria • Contain human genes • Express human proteins • Easy and cheap to grow • Many possibilities

  11. Example: recombinant DNA Insulin

  12. Homework • Read pgs 349-356. Answer Section Assessment questions 1-5