genetic engineering
Download
Skip this Video
Download Presentation
Genetic Engineering

Loading in 2 Seconds...

play fullscreen
1 / 21

Genetic Engineering - PowerPoint PPT Presentation


  • 78 Views
  • Uploaded on

Genetic Engineering. “Amazing Schemes Within Your Genes”. What is genetic engineering?. Moving small pieces of DNA from one organism into another organism. What is recombinant DNA technology?. Same thing as genetic engineering

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Genetic Engineering' - niyati


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
genetic engineering

Genetic Engineering

“Amazing Schemes Within Your Genes”

what is genetic engineering
What is genetic engineering?

Moving small pieces of DNA from one organism into another organism

what is recombinant dna technology
What is recombinant DNA technology?
  • Same thing as genetic engineering
  • Recombinant DNA – made by connecting (splicing) fragments of DNA from different sources
how does recombinant dna work
How does recombinant DNA work?
  • DNA is inserted into an organism
  • Organism uses foreign DNA as if it were its own
what are these new things called
Transgenic organisms

contain recombinant DNA (initial + “foreign”)

What are these new things called?
how do scientists move dna genes to make transgenic organisms
How do scientists move DNA (genes) to make transgenic organisms?
  • Isolate source of foreign DNA to be inserted
  • Join DNA with a vehicle that can transport it
  • Transfer DNA (from chromosomes) into new host
how is dna isolated
How is DNA isolated?

Restriction enzymes are used

what are restriction enzymes
What are restriction enzymes?
  • Proteins that come from bacteria
  • They recognize, bind to and cut DNA into smaller fragments at certain points
  • >200 different enzymes
  • Examples are: EcoRI, HinD III, Bam HI, Bgl II
isolate source of dna
Isolate source of DNA
  • Ex: EcoRI – cuts between G and A whenever it sees CTTAAG

A G G T C T T A A G C T A G C

T C C A G A A T T C G A T C G

isolate source of dna1
Isolate source of DNA
  • Don’t cut straight through both DNA strands
  • Cut is offset by a few bases
  • “Sticky” ends are produced

C T T A A G

G A A T T C

C T T A A G

G A A T T C

isolate source of dna2
Isolate source of DNA

Ex: AluI – cuts between C and G whenever it sees AGCT

A G C T

T C G A

A G C T

T C G A

  • “Blunt” ends are produced
isolate source of dna3
Isolate source of DNA

Sticky Ends

  • DNA can form circle because complementary bases

OR

  • DNA can pair with any other DNA fragment cut by the same enzyme (same bases exposed) = Recombinant DNA
refresher how do scientists move dna genes
REFRESHER: How do scientists move DNA (genes)?
  • Isolate source of DNA

Answer: through Restriction Enzymes

  • Join DNA to a vehicle that can transport it

Answer: ???

  • Transfer DNA (from chromosomes) into target cell

Answer: ???

join dna to a transport vehicle
Join DNA to a transport vehicle

Vector

– means by which DNA from one organism gets to target/host cell

types of vectors
Types of Vectors
  • Mechanical
  • Micropipette inserted into cell
  • Gene gun shoots tiny metal bullet coated w/ DNA
  • Biological
  • Virus
  • Plasmid
what is a plasmid
What is a plasmid?

A small ring of DNA found in bacteria, which only carries genes under extreme circumstances

Both plasmid and foreign DNA are cleaved w/ same restriction enzyme

They are spliced using a “glue” called a ligase (enzyme)

how do we know if foreign dna has been successfully transferred
How do we know if foreign DNA has been successfully transferred?
  • Host cell copies recombinant DNA and its own DNA
  • Clones are created as host continues to divide (all w/ desired DNA)
  • Foreign DNA codes for the production of a specific protein – test the cloned cells to see if they produce protein or not
and the final product is
And the final product is…
  • Newly recombined DNA that undergoes mitosis, producing multiple copies of new DNA
  • Bacteria is considered a good host because it reproduces quickly
ad