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Flow cytometry is a laser-based technique to count and analysis the size, shape and properties of individual cells within a heterogeneous population of cells. Flow cytometry is a widely used approach to phenotype the cells and to assessing the purity of isolated subpopulations. \n\n

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flow cytometry guide

Flow Cytometry Guide

Flow cytometry is a laser-based technique to count and analysis the size, shape and

properties of individual cells within a heterogeneous population of cells. Flow

cytometry is a widely used approach to phenotype the cells and to assessing the

purity of isolated subpopulations. Predominantly, it is measured by the fluorescence

intensity resulted from fluorescent-conjugated antibodies directly recognizing target

proteins, or ligands specifically binding to molecules (such as DNA) within a cell. In

addition, based on the properties of cells, a heterogeneous mixture of biological cells

can be sorted into two or more containers by using a specialized type of flow

cytometry called fluorescence-activated cell sorting (FACS).

Flow cytometry data is extremely quantitative, it provides fast, objective and

simultaneous multi-parameter analysis of single cells as well as physical separation of

cells of particular interest.

Flow cytometer

Suspensions of individual cells from cultured cells, tissues and organisms are

prepared in tubes and then rapidly placed on a flow cytometer. Flow cytometer is

constituted of five main components.

https://www.creative-diagnostics.com/flow-cytometry-guide.htm