Flow Cytometry. Flow Cytometry Basics. Flow cytometry is a technique for counting, examining and sorting microscopic particles (usually cells) suspended in a stream of fluid. Allows simultaneous multi-parametric analysis of the physical and/or chemical characteristics of single cells.
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One unique feature of flow cytometry is that it measures fluorescence per cell or particle. This means that the amount of fluorescent signal detected is directly proportional to the number of fluorochrome molecules on the particle.
By analyzing fluctuations in brightness at each detector it is possible to deduce various facts about the physical and chemical structure of each individual particle (cell).
FSC provides a suitable method of detecting particles greater than a given size independent of their fluorescence and is therefore often used in immuno-phenotyping
When a fluorescent dye is conjugated to a monoclonal antibody, it can be used to identify a particular cell type based on the individual antigenic surface markers of the cell.